Purchase this article with an account.
Hong Hao, Shobi Veleri, Bo Sun, Raman Sood, Paul Liu, Anand Swaroop; The transcription factor neural retina leucine zipper controls photoreceptor-specific expression of Reep6. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2020.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
The transcription factor neural retina leucine zipper (NRL) is essential to specify rod photoreceptor cell fate during retina development, as targeted deletion of Nrl (-/-) in mice led to a complete absence of rod photoreceptors. Nrl -/- rod precursors fail to turn on rod genes and differentiate into functional cone photoreceptors. Previous ChIP-seq analysis of mouse retina revealed NRL binding to the intron of the receptor accessory protein 6 (Reep6). Our goal is to elucidate the mechanism of transcriptional regulation of the Reep6 gene and to understand the molecular mechanism of NRL action.
We performed morpholino experiments in zebra fish to test the functional relevance of Reep6 in retina development. To study the transcriptional regulation of Reep6 gene, we used a combined approach including rapid analysis of cDNA ends (5’-RACE), chromatin immunoprecipitation, luciferase reporter assay, promoter mapping in retina explant and in vivo shRNA analysis.
Knockdown of Reep6 in zebrafish by morpholinos led to microphthalmia. NRL binding to the Reep6 intron enhanced its promoter activity in a luciferase reporter assay and also in a GFP reporter assay in retina explant.
Reep6 expression is controlled by NRL through an enhancer located within its intron. NRL direct target genes serve as excellent candidates for retinal disease gene discovery.
This PDF is available to Subscribers Only