June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Morphologic Dendritic Immune Cells Parameters Reveal Differential Characteristics between the Central and Peripheral Cornea: an In Vivo Confocal Microscopy Normative Data
Author Affiliations & Notes
  • Clara Colon
    Department of Ophthalmology, Ocular Surface Imaging Center, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA
  • Bernardo Cavalcanti
    Department of Ophthalmology, Ocular Surface Imaging Center, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA
    Department of Ophthalmology, Cornea and Refractive Surgery Service, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA
  • Shruti Aggarwal
    Department of Ophthalmology, Ocular Surface Imaging Center, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA
  • Andrea Cruzat
    Department of Ophthalmology, Ocular Surface Imaging Center, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA
    Department of Ophthalmology, Cornea and Refractive Surgery Service, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA
  • Candice Williams
    Department of Ophthalmology, Ocular Surface Imaging Center, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA
  • Douglas Critser
    Department of Ophthalmology & Visual Sciences, Contact Lens Service, University of Iowa, Iowa City, IA
  • Amy Watts
    Contact Lens Service, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA
  • Christine Sindt
    Department of Ophthalmology & Visual Sciences, Contact Lens Service, University of Iowa, Iowa City, IA
  • Pedram Hamrah
    Department of Ophthalmology, Ocular Surface Imaging Center, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA
    Department of Ophthalmology, Cornea and Refractive Surgery Service, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA
  • Footnotes
    Commercial Relationships Clara Colon, None; Bernardo Cavalcanti, None; Shruti Aggarwal, None; Andrea Cruzat, None; Candice Williams, None; Douglas Critser, None; Amy Watts, None; Christine Sindt, alcon (F); Pedram Hamrah, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2063. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Clara Colon, Bernardo Cavalcanti, Shruti Aggarwal, Andrea Cruzat, Candice Williams, Douglas Critser, Amy Watts, Christine Sindt, Pedram Hamrah; Morphologic Dendritic Immune Cells Parameters Reveal Differential Characteristics between the Central and Peripheral Cornea: an In Vivo Confocal Microscopy Normative Data. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2063.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To determine the morphology of dendritic immune cells (DC) in the central and four peripheral quadrants in corneas of normal subjects.

Methods: Eighty-five normal non-contact lens wearers (85 eyes) were prospectively enrolled and underwent laser in vivo confocal microscopy (IVCM; HRT3/RCM) of the central cornea, as well as inferior, nasal, superior and temporal quadrants. Slit-lamp examination was performed to confirm lack of ocular disease. Baseline images were assessed for DC morphology and density by two masked observers. Morphology was assessed by number of dendrites per cell, area of DC and DC field. Statistical analysis was performed with ANOVA/Bonferroni to compare the differences between corneal areas and intraclass coefficient was used to assess interobserver reproducibility.

Results: The mean age of subjects was 31.4 years (range 20 to 69). IVCM revealed the presence of central (35.6±4.3 cells/mm2) and peripheral (74.1±4.5) corneal DC in all areas of all subjects. The number of dendrites, area of DC, and DC field centrally were 2.4±0.04 n/cell, 71.6±4.3 µm2, and 304.2±19.2 µm2 respectively. Number of dendrites (3.2±0.07 inferior, 2.9±0.03 nasal, 3.0±0.03 superior, 2.9±0.03 temporal), area of DC (134.6±3.5, 115.3±3.4, 117.0±3.5, 121.2±3.9), and DC field area (684.4±19.1, 586.2±19.7, 587.7±17.4, 601±18.6), were found to be significantly larger in all 4 peripheral quadrants (p<0.001). While the peripheral areas had larger DC in general, the inferior quadrant demonstrated the largest area of DC and DC field, as well as the highest dendrite numbers. Good interobserver reproducibility coefficients were found for DC area (0.83; 0.77-0.91 95%CI), number of dendrites (0.97; 0.95-0.99), and DC coverage area (0.94; 0.85-0.98).

Conclusions: IVCM revealed significant difference in morphology of peripheral versus central corneal DC. These changes suggest increased DC activation in peripheral cornea. The morphological parameters may be used to detect early changes in the corneal inflammatory status. Further, they may provide additional data to investigate cell activation state for corneal disease and monitor response to anti-inflammatory therapy. With the increased use of IVCM to detect subclinical inflammatory changes, the normative data may serve as the basis for future clinical studies and trials.

Keywords: 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 557 inflammation • 550 imaging/image analysis: clinical  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×