June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Model of allergic keratoconjunctivitis - Stat6 signaling in chronic inflammation
Author Affiliations & Notes
  • Michael Conwell
    Ophthalmology, Glick Eye Institute, Indianapolis, IN
  • Sonia DaSilva-Arnold
    Dermatology, Indiana University, Indianapolis, IN
  • Na Luo
    Ophthalmology, Glick Eye Institute, Indianapolis, IN
  • Wei Li
    Xiamen Eye Institute, Xiamen University, Xiamen, China
  • Jeffery Travers
    Dermatology, Indiana University, Indianapolis, IN
  • Yang Sun
    Ophthalmology, Glick Eye Institute, Indianapolis, IN
    Dermatology, Indiana University, Indianapolis, IN
  • Footnotes
    Commercial Relationships Michael Conwell, None; Sonia DaSilva-Arnold, None; Na Luo, None; Wei Li, None; Jeffery Travers, None; Yang Sun, NIH (F), American Glaucoma Society (R), Knights Templar Eye Foundation (R), Reeves Foundation (R)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2081. doi:
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    • Get Citation

      Michael Conwell, Sonia DaSilva-Arnold, Na Luo, Wei Li, Jeffery Travers, Yang Sun; Model of allergic keratoconjunctivitis - Stat6 signaling in chronic inflammation. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2081.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

The purpose of this study is to characterize the corneal inflammatory lesions found in the Stat6VT mouse as a model for atopic dermatitis. We hypothesize that the constitutive activation of Stat6 results in corneal neovascularization and pannus formation.

 
Methods
 

SKH1-HrHr wild-type mice and mice which express an integrally active Stat6 (Stat6VT), V547A/T548A, (N=20, 24 respectively) where examined for the development of keratoconjunctivitis. Corneas were removed and examined by H&E and PAS staining. Immunoblot analysis was performed with Interleukin-4, Interferon gamma, and CD3. Steroid treatment with clobetasol was performed on affected lesions.

 
Results
 

Compared to wild-type, Stat6VT transgenic mice exhibited increased frequency of corneal neovascularization (N=24, P<0.05). The Stat6VT transgenic mice demonstrated corneal infiltrate as well as neovascularization (N=5, P<0.05). Real-time PCR analyses performed on the mRNA from both the wild type and Stat6VT mice yielded elevated levels of Th2 cytokines isolated from the Stat6VT mice (N=5,5 respectively P<0.05). We showed that clobetasol treatment slowed the development of periocular and corneal lesions.

 
Conclusions
 

Stat6 signaling may play a role in the pathogenesis of chronic ocular inflammation. IL-4 may mediate corneal inflammation in allergic keratoconjunctivitis.

 
 
Figure 1. Corneal infiltrates in STAT6VT mice. (A) WT and (B) STAT6VT mice cornea were stained by H&E. Scale bar represents 10 microns.
 
Figure 1. Corneal infiltrates in STAT6VT mice. (A) WT and (B) STAT6VT mice cornea were stained by H&E. Scale bar represents 10 microns.
 
Keywords: 474 conjunctiva • 557 inflammation • 480 cornea: basic science  
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