June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Role of IL-7 in Corneal Lymphangiogenesis
Author Affiliations & Notes
  • Tatiana Ecoiffier
    Vision Science, University of California, Berkeley, School of Optometry, Center for Eye Disease and Development, Berkeley, CA, 94720, USA, CA
  • Maria Iolyeva
    Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland
  • Cornelia Halin
    Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland
  • Lu Chen
    Vision Science, University of California, Berkeley, School of Optometry, Center for Eye Disease and Development, Berkeley, CA, 94720, USA, CA
  • Footnotes
    Commercial Relationships Tatiana Ecoiffier, None; Maria Iolyeva, None; Cornelia Halin, None; Lu Chen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2088. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Tatiana Ecoiffier, Maria Iolyeva, Cornelia Halin, Lu Chen; Role of IL-7 in Corneal Lymphangiogenesis. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2088.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Interleukin-7 (IL-7) is an immunoregulatory cytokine that have been recently identified on lymphatic endothelial cells. The purpose of this study was to investigate the in vivo effect of IL-7 on corneal lymphangiogenesis (LG, the growth of new lymphatic vessels).

Methods: Uniform slow-release IL-7 pellets were prepared by mixing IL-7 (Peprotech, Rocky Hill, NJ) or appropriate control with sucralfate and hydron (Sigma Aldrich, St. Louis, MO). A micropocket was surgically created in BALB/c mouse cornea and a pellet was implanted 1.0 mm apart from the limbal vascular arcade. Corneas were harvested after 2 weeks and stained with LYVE-1, lymphatic specific marker. Percentage LG covered area under both control and IL-7 treated conditions were evaluated using NIH Image J software.

Results: Pellets containing IL-7 induced a 6.4 fold increase in the corneal area covered by lymphatic vessels compared to the control condition (p < 0.05).

Conclusions: Our data demonstrate that IL-7 is a strong stimulator of lymphangiogenesis in vivo. This study will shed some new light on our understanding of corneal lymphangiogenesis and potentially promotes the development of novel therapeutic strategies for lymphatic related diseases occurring inside and outside the eye.

Keywords: 609 neovascularization • 480 cornea: basic science  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×