June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Impaired angiogenic response in cornea by lacking TRPV1 in mice
Author Affiliations & Notes
  • Katsuo Tomoyose
    Wakayama Medical University, Wakayama, Japan
  • Yuka Okada
    Wakayama Medical University, Wakayama, Japan
  • Takayoshi Sumioka
    Wakayama Medical University, Wakayama, Japan
  • Kumi Shirai
    Wakayama Medical University, Wakayama, Japan
  • Shizuya Saika
    Wakayama Medical University, Wakayama, Japan
  • Footnotes
    Commercial Relationships Katsuo Tomoyose, None; Yuka Okada, None; Takayoshi Sumioka, None; Kumi Shirai, None; Shizuya Saika, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2092. doi:
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    • Get Citation

      Katsuo Tomoyose, Yuka Okada, Takayoshi Sumioka, Kumi Shirai, Shizuya Saika; Impaired angiogenic response in cornea by lacking TRPV1 in mice. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2092.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigate the effects of loss of Transient receptor potential (TRP)V1 in the development of neovascularization (NV) in a corneal stroma in mice. TRP channels are polymodal receptors that are activated by a host of stimuli to mediate sensory transduction.

Methods: Corneal NV from the limbal vessels were induced by cauterization of the central cornea of an eye by using disposable tool of Optemp. WT and TRPV1-null (KO) mice (n = 16 in each genotype) were used and killed at day 3 and 7. The eye was enucleated and processed or cryosectioning for histology and immunohistochemistry. To examine the expression of angiogenic growth factors in vivo, centrally cauterized cornea (n = 40 in each of WT or KO group) was excised at day 3. Total RNA was extracted from samples and processed for real-time RT-PCR for VEGF, TGFb1.

Results: The development of CD31-labeled new vessels from the limbus toward the center of the cornea was impaired in KO mice as compared with WT mice at day 3 days, but not at day 7. Expression of mRNAs of VEGF and TGFb1 was significantly less in a KO cornea as compared with a WT cornea at day 3.

Conclusions: TRPV1 signal is involved in the development of cauterization-induced NV in corneal strom via modulation of angiogenic gene expression in the affected tissue.

Keywords: 609 neovascularization • 765 wound healing  
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