June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
MicroRNA-184 Regulates Corneal Lymphangiogenesis
Author Affiliations & Notes
  • Lu Chen
    Center for Eye Disease & Development, Program in Vision Science and School of Optometry, University of California, Berkeley, CA
  • Sammy Grimaldo
    Center for Eye Disease & Development, Program in Vision Science and School of Optometry, University of California, Berkeley, CA
  • Tatiana Ecoiffier
    Center for Eye Disease & Development, Program in Vision Science and School of Optometry, University of California, Berkeley, CA
  • Don Yuen
    Center for Eye Disease & Development, Program in Vision Science and School of Optometry, University of California, Berkeley, CA
  • Footnotes
    Commercial Relationships Lu Chen, None; Sammy Grimaldo, None; Tatiana Ecoiffier, None; Don Yuen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2095. doi:
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    • Get Citation

      Lu Chen, Sammy Grimaldo, Tatiana Ecoiffier, Don Yuen; MicroRNA-184 Regulates Corneal Lymphangiogenesis. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2095.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: MicroRNAs are a class of small non-coding RNAs that negatively regulate gene expression by binding to complimentary sequences of target messenger RNAs. Their roles in the cornea still remain largely unknown. Here we present our new findings on the anti-lymphangiogenesis function of microRNA-184 in the cornea during an inflammatory response in vivo.

Methods: The murine in vivo suture placement model was used to induce corneal inflammatory lymphangiogenesis (LG). Mice were randomly selected to receive subconjunctival injections of either synthetic microRNA-184 mimics or control twice a week after the procedure. Whole-mount corneas were sampled and stained with LYVE-1, the lymphatic marker, for immunofluorescent microscopic assays. Results were analyzed by NIH-ImageJ software.

Results: Compared to the control condition, the lymphatic invasion area was significantly reduced after the treatment of MicroRNA-184 mimics in the inflamed cornea.

Conclusions: MicroRNA-184 suppresses corneal lymphangiogenesis. Its further investigation may provide novel therapies for lymphatic related disorders in the cornea, such as inflammation and transplant rejection.

Keywords: 609 neovascularization • 480 cornea: basic science • 555 immunomodulation/immunoregulation  
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