June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Live Imaging of Lymphatic Valve Formation after Corneal Transplantation
Author Affiliations & Notes
  • Gyeong Jin Kang
    Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, CA
  • Tatiana Ecoiffier
    Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, CA
  • Young-Kwon Hong
    Department of Surgery, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA
  • Lu Chen
    Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, CA
  • Footnotes
    Commercial Relationships Gyeong Jin Kang, None; Tatiana Ecoiffier, None; Young-Kwon Hong, None; Lu Chen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2096. doi:
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    • Get Citation

      Gyeong Jin Kang, Tatiana Ecoiffier, Young-Kwon Hong, Lu Chen; Live Imaging of Lymphatic Valve Formation after Corneal Transplantation. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2096.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The lymphatic pathway is a major mediator for transplant rejection. Most recently, we have provided the first evidence on lymphatic valve formation during corneal inflammatory lymphangiogenesis (Truong et al. 2011). The purpose of this real-time in vivo study is to investigate the time course and pattern of lymphangiogenesis as well as lymphatic valve formation induced by corneal transplantation with our newly developed live imaging system and Prox-1-GFP mice.

Methods: Standard orthotopic corneal transplantation was performed with Prox1-GFP mice as recipients. Corneal grafts of the same mice were continuously observed by the live imaging system for longitudinal analysis. Prox-1 positive lymphatic vessels and valves were evaluated at both limbal and corneal areas.

Results: Prox-1 positive lymphatic vessels and valves were formed after corneal transplantation. As corneal lymphangiogenesis proceeded, more valves were observed inside vessel cavities. Furthermore, lymphatic valvulogenesis was initiated inside limbal vessels before spreading into the central cornea.

Conclusions: We have shown, for the first time, corneal transplantation induces both lymphangiogenesis and valvulogenesis in vivo and in real time. Further investigation on this new phenomenon may reveal new mechanisms underlying transplant rejection. Since the lymphatic system plays an important role in many other functions, this study may also offer new insights to our understanding and treatment of other lymphatic related disorders outside the eye.

Keywords: 480 cornea: basic science • 741 transplantation • 609 neovascularization  
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