June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Analysis of VEGFR-1 Isoforms in Pax6-deficient Mice Corneas
Author Affiliations & Notes
  • Phillip Moore
    Dept Small Animal Med/Surg, College Vet Med Univ Georgia, Athens, GA
  • Peter Accola
    Dept Small Animal Med/Surg, College Vet Med Univ Georgia, Athens, GA
  • Barbara Artelt
    Dept Small Animal Med/Surg, College Vet Med Univ Georgia, Athens, GA
  • Megan Aarnio
    Dept Small Animal Med/Surg, College Vet Med Univ Georgia, Athens, GA
  • James Lauderdale
    Department of Cellular Biology, Franklin College of Arts and Sciences, The University of Georgia, Athens, GA
  • Footnotes
    Commercial Relationships Phillip Moore, None; Peter Accola, None; Barbara Artelt, None; Megan Aarnio, None; James Lauderdale, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2101. doi:
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    • Get Citation

      Phillip Moore, Peter Accola, Barbara Artelt, Megan Aarnio, James Lauderdale; Analysis of VEGFR-1 Isoforms in Pax6-deficient Mice Corneas. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2101.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To identify VEGFR-1 isoforms in the cornea of Pax6-deficient mice.

Methods: The limbal region, central cornea and conjunctiva of Pax6 +/- mice were compared to wild type mice for the presence of VEGFR-1 and sVEGFR-1. Immunostaining was performed to wild type (n=5) and Pax6 +/- mice (n= 5) with three antibodies: one antibody to the N-terminus that detected both membrane and soluble VEGFR-1; one antibody to the C-terminus that detected the membrane bound form of VEGFR-1; and one antibody that specifically detected the soluble form of VEGFR-1. Immunoblotting was performed on protein extracts prepared separately from the cornea and conjunctiva of wild type and Pax6 +/- mice.

Results: Immunostaining revealed that both VEGFR-1 and sVEGFR-1 are present in the cornea of both wild type and Pax6 +/- mice. Immunoblotting revealed that both VEGFR-1 and sVEGFR-1 are present in the conjunctiva and central cornea of wild type and Pax6 +/- mice. Interestingly, this analysis also revealed VEGFR-1 isoforms that appeared to be specific to Pax6 +/- corneas.

Conclusions: Our results are in contradiction to previous reports that sVEGFR-1 is deficient in Pax6 +/- corneas. The presence of sVEGFR-1 and VEGFR-1 isoforms specific to Pax6 +/- corneas suggests that a deficiency in sVEGFR-1 is not the cause for corneal neovascularization in Pax6 +/- mice.

Keywords: 480 cornea: basic science • 482 cornea: epithelium • 609 neovascularization  
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