June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Resolvin D1 Receptor Activation Counter-regulates H1 histamine receptors in human and rat conjunctival goblet cells
Author Affiliations & Notes
  • Robin Hodges
    Schepens Eye Research Institute, Massachusetts Eye and Ear, Boston, MA
    Department of Ophthalmology, Harvard Medical School, Boston, MA
  • Dayu Li
    Schepens Eye Research Institute, Massachusetts Eye and Ear, Boston, MA
    Department of Ophthalmology, Harvard Medical School, Boston, MA
  • Richard Carozza
    Schepens Eye Research Institute, Massachusetts Eye and Ear, Boston, MA
    Department of Ophthalmology, Harvard Medical School, Boston, MA
  • Marie Shatos
    Schepens Eye Research Institute, Massachusetts Eye and Ear, Boston, MA
    Department of Ophthalmology, Harvard Medical School, Boston, MA
  • Nan Chiang
    Center Center for Experimental Therapeutics and Reperfusion Injury, Harvard Institutes of Medicine, Harvard Medical School, Boston, MA
    Department of Anesthesiology, Preoperative, and Pain Management, Brigham and Women's Hospital, Boston, MA
  • Charles Serhan
    Center Center for Experimental Therapeutics and Reperfusion Injury, Harvard Institutes of Medicine, Harvard Medical School, Boston, MA
    Department of Anesthesiology, Preoperative, and Pain Management, Brigham and Women's Hospital, Boston, MA
  • Darlene Dartt
    Schepens Eye Research Institute, Massachusetts Eye and Ear, Boston, MA
    Department of Ophthalmology, Harvard Medical School, Boston, MA
  • Footnotes
    Commercial Relationships Robin Hodges, None; Dayu Li, None; Richard Carozza, None; Marie Shatos, None; Nan Chiang, None; Charles Serhan, Resolvyx Pharma (I), BWH- Partners (P); Darlene Dartt, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2114. doi:
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      Robin Hodges, Dayu Li, Richard Carozza, Marie Shatos, Nan Chiang, Charles Serhan, Darlene Dartt; Resolvin D1 Receptor Activation Counter-regulates H1 histamine receptors in human and rat conjunctival goblet cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2114.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To determine the interactions between H1 histamine receptor (H1R) and GPR32, a GPCR receptor for the proresolution mediator, resolvin D1 (RvD1).

Methods: Rat conjunctival goblet cells were grown in culture. Chinese hamster ovary (CHO) cells were transfected with human H1R and GPR32 receptor. Intracellular [Ca2+] ([Ca2+]i) was measured in cells loaded with the calcium indicator dye fura 2.

Results: In rat goblet cells, the H1R agonist histamine dimaleate significantly increased [Ca2+]i by 327.2 ± 71.5 nM. Exposure to RvD1 significantly inhibited this response to 92.6 ± 21.5 nM. The protein kinase C inhibitor Ro-317549 and an inhibitory peptide of β adrenergic receptor kinase 1 (βARK1) reversed the inhibition of RvD1 on the histamine dimaleate stimulated increase in [Ca2+]i to 197.2 ± 46.3 and 352.7 ± 89.6 nM, respectively. Similar results were obtained in CHO cells transfected with human H1R and human GPR32 receptor.

Conclusions: We conclude that RvD1 uses PKC and βARK1 to counter-regulate the H1R and inhibit its actions.

Keywords: 474 conjunctiva • 557 inflammation • 486 cornea: tears/tear film/dry eye  
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