June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Effect of Cyclosporin A (Cys A) to the Matrix Metalloproteinase (MMP)-3 and MMP-13 on the Migration of Cultured Pterygial Fibroblast Cells
Author Affiliations & Notes
  • Young Jeung Park
    Ophthalmology, Cheil eye hospital, Daegu, Republic of Korea
  • Yeoun-Hee Kim
    Ophthalmology, Cheil eye hospital, Daegu, Republic of Korea
    Biology, Kyungpook National University, Daegu, Republic of Korea
  • Se-Hyung Lee
    Biology, Kyungpook National University, Daegu, Republic of Korea
  • Jae-Chang Jung
    Biology, Kyungpook National University, Daegu, Republic of Korea
  • Kyoo Won Lee
    Ophthalmology, Cheil eye hospital, Daegu, Republic of Korea
  • Footnotes
    Commercial Relationships Young Jeung Park, None; Yeoun-Hee Kim, None; Se-Hyung Lee, None; Jae-Chang Jung, None; Kyoo Won Lee, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2117. doi:
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      Young Jeung Park, Yeoun-Hee Kim, Se-Hyung Lee, Jae-Chang Jung, Kyoo Won Lee; Effect of Cyclosporin A (Cys A) to the Matrix Metalloproteinase (MMP)-3 and MMP-13 on the Migration of Cultured Pterygial Fibroblast Cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2117.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigate the effects of matrix metalloproteinase (MMP) 3 and MMP-13 expression in cultured human pterygial fibroblast cells according to the concentration and exposure time of Cyclosporine A (Cys A) after being scratched.

Methods: Scratched-human pterygial fibroblast cells were exposed to a Cys A concentrations of 1 µg/ml (0.0001%) and 100 µg/ml (0.01%) for 3 or 10 minutes and then cells were washed with D-PBS and re-incubated or exposed to a MMP-3 specific inhibitor (MMP-3 VII inhibitor) or MMP-9/13 inhibitor with serum depletion DMEM-F-12 (1:1) media for 48 hours. Cells were lysed for Western blotting or extracted mRNA for RT-PCR. Supernatant media were used to perform TCA precipitation for Western blotting. MMP-3 and MMP-13 expression of fibroblast after the stimulation were studied.

Results: The cellular migrations were markedly suppressed by pre-treatment with Cys A in a time- and dose- dependent manner on the cultured pterygial fibroblast cells (P < 0.01). Interestingly, this migration was also blocked by MMP-3 specific inhibitor or MMP-9-13 inhibitor (P < 0.01). Furthermore, pre-treatment of Cys A reduced MMP-3 mRNA (P < 0.01) and MMP-13 protein (P < 0.02) expression level.

Conclusions: Previous studies reported that the expression of several types of MMPs was increased in pterygium, and Cys A can be effective in preventing recurrences after primary pterygium surgery. Our results suggested that Cys A inhibited cell migration after being scratched by reducing expression of MMPs. These findings reveal therapeutic potential for pterygium progression.

Keywords: 474 conjunctiva • 489 cyclosporine • 665 pterygium  
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