June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Lymphatic and Blood Vessel Density in Human Conjunctiva Following Glaucoma Surgery
Author Affiliations & Notes
  • Mirela Krasniqi
    Ophthalmology, Summa-Health System, Akron, OH
  • Heidi Rassavong
    Ophthalmology, Summa-Health System, Akron, OH
  • Rachida Bouhenni
    Ophthalmology, Summa-Health System, Akron, OH
  • Jeffrey Dunmire
    Ophthalmology, Summa-Health System, Akron, OH
  • Sami Al Shahwan
    Ophthalmology, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia
  • Ibrahim Al Jadaan
    Ophthalmology, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia
  • Hind Alkatan
    Ophthalmology, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia
  • Deepak Edward
    Ophthalmology, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia
    Ophthalmology, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD
  • Footnotes
    Commercial Relationships Mirela Krasniqi, None; Heidi Rassavong, None; Rachida Bouhenni, None; Jeffrey Dunmire, None; Sami Al Shahwan, None; Ibrahim Al Jadaan, None; Hind Alkatan, None; Deepak Edward, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2121. doi:
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      Mirela Krasniqi, Heidi Rassavong, Rachida Bouhenni, Jeffrey Dunmire, Sami Al Shahwan, Ibrahim Al Jadaan, Hind Alkatan, Deepak Edward; Lymphatic and Blood Vessel Density in Human Conjunctiva Following Glaucoma Surgery. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2121.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigate the lymphatic vascular microvessel density (LVD) and the blood vascular microvessel density (MVD) and their distribution in excised leaking blebs after mitomycin C (MMC) trabeculectomy and normal conjunctiva specimens.

Methods: LVD and MVD in normal human conjunctiva (n=7) and excised blebs in the hypocellular stroma and peribleb tissue (conjunctiva adjacent to hypocellular bleb tissue, n=7) was evaluated by immunohistochemistry using antibodies raised against Lymphatic Vessel Endothelial Receptor 1 (LYVE-1, lymphatic vessels) and CD34 (blood vessels). LVD and MVD counts were performed by light microscopy in 5 fields at 20X magnification by three observers. Differences were determined using Mann Whitney U test (p<0.05 was considered significant).

Results: The LVD and MVD were significantly reduced to absent in the hypocellular conjunctival stroma of the excised blebs compared to normal conjunctiva (21.42 vs. 1.16, p<0.002 and 24.28 vs. 1, p< 0.008 respectively). The LVD and MVD was also reduced (2 to 2.5 fold) in the peribleb stroma when compared to normal conjunctiva specimens.

Conclusions: Reduced LCD and MVD in the hypocellular stroma and peribleb stroma may play a role in an altered immune response in leaking blebs leading to an increased risk of blebitis.

Keywords: 474 conjunctiva • 554 immunohistochemistry • 436 blood supply  
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