June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Corneal endothelial cells provide evidence of accelerated cellular senescence associated with HIV infection: a case-control study
Author Affiliations & Notes
  • Sophia Pathai
    International Centre for Eye Health, London School of Hygiene & Tropical Medicine, London, United Kingdom
    Desmond Tutu HIV Centre, University of Cape Town, Cape Town, South Africa
  • Stephen Lawn
    Desmond Tutu HIV Centre, University of Cape Town, Cape Town, South Africa
    Dept of Clinical Research, London School of Hygiene & Tropical Medicine, London, United Kingdom
  • Paul Shiels
    Dept of Epigenetics, Institute of Cancer Sciences, University of Glasgow, Glasgow, United Kingdom
  • Helen Weiss
    MRC Tropical Epidemiology Group, London School of Hygiene & Tropical Medicine, London, United Kingdom
  • Colin Cook
    Dept of Ophthalmology, University of Cape Town, Cape Town, South Africa
  • Robin Wood
    Desmond Tutu HIV Centre, University of Cape Town, Cape Town, South Africa
  • Clare Gilbert
    International Centre for Eye Health, London School of Hygiene & Tropical Medicine, London, United Kingdom
  • Footnotes
    Commercial Relationships Sophia Pathai, None; Stephen Lawn, None; Paul Shiels, Pathfinder Cell Therapy (F), Sanofi Aventis (C); Helen Weiss, None; Colin Cook, None; Robin Wood, None; Clare Gilbert, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2200. doi:
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    • Get Citation

      Sophia Pathai, Stephen Lawn, Paul Shiels, Helen Weiss, Colin Cook, Robin Wood, Clare Gilbert; Corneal endothelial cells provide evidence of accelerated cellular senescence associated with HIV infection: a case-control study. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2200.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Cellular senescence may be a key factor in HIV-related premature biological aging. We assessed features of the corneal endothelium that are known to be associated with biological aging, and cellular senescence markers in HIV-infected adults.

Methods: Case-control study of 242 HIV-infected adults and 249 matched controls. Using specular microscopy, the corneal endothelium was assessed for features of aging (low endothelial cell density [ECD], high variation in cell size, and low hexagonality index). Data were analysed by multivariable regression. CDKN2A (a cell senescence mediator) and 8-hydroxy-2′-deoxyguanosine (an oxidative DNA damage marker) were measured in peripheral blood leukocytes.

Results: The median age of both groups was 40 years. Among HIV-infected adults, 88% were receiving antiretroviral therapy (ART); their median CD4 count was 468 cells/μL. HIV infection was associated with increased odds of variation in cell size (OR=1.67; 95%CI: 1.00-2.78, p=0.04). Among HIV-infected participants, low ECD was independently associated with current CD4 count <200 cells/μL (OR=2.77; 95%CI: 1.12-6.81, p=0.03). In participants on ART with undetectable viral load, CDKN2A expression and 8-OHDG levels were higher in those with accelerated aging, as reflected by lower ECD.

Conclusions: The corneal endothelium shows features consistent with HIV-related accelerated senescence, especially among those with poor immune recovery.

Keywords: 415 AIDS/HIV • 481 cornea: endothelium • 413 aging  
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