June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Three-Dimensional (3-D) Histomorphomety of Normal Human Optic Nerve Head (ONH) Connective Tissue at physiologic and elevated IOP
Author Affiliations & Notes
  • Lan Wang
    Ophthal-CEFH, Univ of Alabama at Birmingham, Birmingham, AL
  • Hongli Yang
    Discoveries in Sight, Devers Eye Institute, Legacy Research Institute, Portland, OR
  • Massimo Fazio
    Ophthal-CEFH, Univ of Alabama at Birmingham, Birmingham, AL
  • Brandon Smith
    Ophthal-CEFH, Univ of Alabama at Birmingham, Birmingham, AL
  • Chad Cheetham
    Ophthal-CEFH, Univ of Alabama at Birmingham, Birmingham, AL
  • J Crawford Downs
    Ophthal-CEFH, Univ of Alabama at Birmingham, Birmingham, AL
  • Juan Reynaud
    Discoveries in Sight, Devers Eye Institute, Legacy Research Institute, Portland, OR
  • Howard Lockwood
    Discoveries in Sight, Devers Eye Institute, Legacy Research Institute, Portland, OR
  • Claude Burgoyne
    Discoveries in Sight, Devers Eye Institute, Legacy Research Institute, Portland, OR
  • Christopher Girkin
    Ophthal-CEFH, Univ of Alabama at Birmingham, Birmingham, AL
  • Footnotes
    Commercial Relationships Lan Wang, None; Hongli Yang, None; Massimo Fazio, None; Brandon Smith, None; Chad Cheetham, None; J Crawford Downs, None; Juan Reynaud, None; Howard Lockwood, None; Claude Burgoyne, Heidelberg Engineering (F), Heidelberg Engineering (C); Christopher Girkin, SOLX (F), Heidelberg Engineering (F)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2259. doi:
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    • Get Citation

      Lan Wang, Hongli Yang, Massimo Fazio, Brandon Smith, Chad Cheetham, J Crawford Downs, Juan Reynaud, Howard Lockwood, Claude Burgoyne, Christopher Girkin; Three-Dimensional (3-D) Histomorphomety of Normal Human Optic Nerve Head (ONH) Connective Tissue at physiologic and elevated IOP. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2259.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To compare the difference of normal human ONH connective tissue structure at physiologic and elevated IOP by 3D histomorphometry.

 
Methods
 

38 eyes from 19 human donors (54±17 years old), collected within 6 hours postmortem underwent aldehyde fixation at 10mmHg IOP in both eyes (7 pairs) or 10 mmHg in the right eye and 45 mmHg in the left eye (12 pairs). The ONH and peripapillary sclera were trephinated and serial sectioned using an automated microtome-based system that yielded serial fluorescent images of the block face as we cut through the ONH at 1.5 μm section thickness; the autofluorescent images were aligned and stacked into a 3D ONH reconstruction with 1.5x1.5x1.5 μm voxel resolution (Downs et al. ISER 2010). The ONH and peripapillary scleral anatomy was delineated in custom software (Downs, Yang et al. IOVS 2007). The 80 Bruch’s membrane opening (BMO) points were used to establish a zero reference plane for all measurements. The BMO radius, neural canal depth, lamina cribrosa thickness and depth, and scleral thickness were calculated and analyzed using mixed effects models with generalized estimation equations.

 
Results
 

In adjusted models, IOP elevation from 10 to 45 mmHg resulted in a significant decrease in the posterior scleral canal opening radius (p = 0.028) and posterior laminar insertion radius (p = 0.026). No other significant differences in morphometric parameters were detected (Table 1).

 
Conclusions
 

While no significant changes in laminar position were seen, contraction of the posterior neural canal occurs in response to IOP elevation in normal human donor eyes.

 
 
ASCO: Anterior Sclera Canal Opening; ALI: Anterior Lamina Insertion; PSCO: Posterior Sclera Canal Opening; PLI: Posterior Lamina Insertion. * Significantly different at p<0.05
 
ASCO: Anterior Sclera Canal Opening; ALI: Anterior Lamina Insertion; PSCO: Posterior Sclera Canal Opening; PLI: Posterior Lamina Insertion. * Significantly different at p<0.05
 
Keywords: 629 optic nerve • 551 imaging/image analysis: non-clinical • 613 neuro-ophthalmology: optic nerve  
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