June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Reproducibility of the measurement of Optic Nerve Head Hemoglobin levels
Author Affiliations & Notes
  • Carmen Mendez-Hernandez
    Glaucoma, Instituto de Investigación Sanitaria San Carlos (IdISSC) Fundación de Investigación Biomédica del Hospital Clínico San Carlos, Madrid, Spain
  • Ignacio Rodriguez-Uña
    Glaucoma, Instituto de Investigación Sanitaria San Carlos (IdISSC) Fundación de Investigación Biomédica del Hospital Clínico San Carlos, Madrid, Spain
  • Paula Arribas Pardo
    Glaucoma, Instituto de Investigación Sanitaria San Carlos (IdISSC) Fundación de Investigación Biomédica del Hospital Clínico San Carlos, Madrid, Spain
  • Federico Saenz-Frances
    Glaucoma, Instituto de Investigación Sanitaria San Carlos (IdISSC) Fundación de Investigación Biomédica del Hospital Clínico San Carlos, Madrid, Spain
  • Manuel González-de-la-Rosa
    Hospital Universitario de Canarias., La Laguna, Spain
  • Jose Martinez de la Casa
    Glaucoma, Instituto de Investigación Sanitaria San Carlos (IdISSC) Fundación de Investigación Biomédica del Hospital Clínico San Carlos, Madrid, Spain
  • Enrique Santos-Bueso
    Glaucoma, Instituto de Investigación Sanitaria San Carlos (IdISSC) Fundación de Investigación Biomédica del Hospital Clínico San Carlos, Madrid, Spain
  • Julian Garcia-Feijoo
    Glaucoma, Instituto de Investigación Sanitaria San Carlos (IdISSC) Fundación de Investigación Biomédica del Hospital Clínico San Carlos, Madrid, Spain
  • Footnotes
    Commercial Relationships Carmen Mendez-Hernandez, None; Ignacio Rodriguez-Uña, None; Paula Arribas Pardo, None; Federico Saenz-Frances, None; Manuel González-de-la-Rosa, Haag Streit (P), Oculus (P); Jose Martinez de la Casa, None; Enrique Santos-Bueso, None; Julian Garcia-Feijoo, Trancend (C), Ivantis (C), Glaukos (C), MSD (C), Allergan (F), Pfizer (F), Alcon (C), Sensimed (F), Sylentis (F), Bausch and Lomb (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2287. doi:
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      Carmen Mendez-Hernandez, Ignacio Rodriguez-Uña, Paula Arribas Pardo, Federico Saenz-Frances, Manuel González-de-la-Rosa, Jose Martinez de la Casa, Enrique Santos-Bueso, Julian Garcia-Feijoo, PI09/90933 of the Instituto de Salud Carlos III grants. Red Temática de Investigación Cooperativa ISCIII: RD07/062; Reproducibility of the measurement of Optic Nerve Head Hemoglobin levels. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2287.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To evaluate the intraobserver, interobserver, within and between-session reproducibility of the measurement of Optic Nerve Head (ONH) Hemoglobin levels using colour analysis by Laguna ONhE (Optic Nerve Hemoglobine) program.

Methods: Observational prospective study of 29 eyes (11 hypertensive and glaucomatous; 18 healthy eyes). Two investigators obtained two retinographies (Canon non-mydriatic retinal camera CD-DGi, Canon Inc.,Tokyo, Japan) in two testing sessions three weeks apart and analyzed the images using the Laguna ONhE (Optic Nerve Hemoglobine). The following parameters were quantified: ONH Hemoglobin concentration in its whole extent and in vertical disk diameter, disc-cup ratio and GDF (Glaucoma Discriminant Function). Agreement was illustrated using Bland-Altman plots and reproducibility was assessed comparing the intraclass correlation coefficients (ICC).

Results: In session 1, investigator 1 found mean levels of ONH Hemoglobin of 67.94 +/- 8.70% in healthy eyes and of 57.90+/-5.36% in hypertensive and glaucomatous eyes. Correspondent values for investigator 2 were 68.27 +/- 8.52% and 57.83 +/-4.88%, respectively. ONH Hemoglobin concentration measurements, in its whole as well as in the vertical diameter, showed the highest ICCs (All above 0.9). Variability was greater for GFD (ICC>0.8) and cup-disc ratio (ICC>0.71).

Conclusions: The measurement of ONH Hemoglobin concentration using the Laguna ONhE program shows high reproducibility both in glaucomatous and non-glaucomatous ONHs.

Keywords: 549 image processing • 610 nerve fiber layer • 628 optic flow  
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