June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Endothelial Progenitor Cells (EPCs) Detection In The Peripheral Blood Of Young Diabetic Patients with Type 1 Diabetes Mellitus (T1DM)- A Pilot Study
Author Affiliations & Notes
  • Chrysanthi Tsika
    Department of Neurology and Sensory Organs, Eye CLinic of University Hospital of Heraklion, University of Crete, Medical School, Heraklion, Greece
  • Zoi Kapsala
    Department of Neurology and Sensory Organs, Eye CLinic of University Hospital of Heraklion, University of Crete, Medical School, Heraklion, Greece
  • Charalampos Pontikoglou
    Department of Internal Medicine, Hematology Clinic of the Univesity Hospital of Heraklion, University of Crete, Medical School, Heraklion, Greece
  • Vassiliki Louvari
    Endocrinology & Diabetes, Department of Pediatrics, University of Crete, Medical School, Heraklion, Greece
  • Dimitrios Mamoulakis
    Endocrinology & Diabetes, Department of Pediatrics, University of Crete, Medical School, Heraklion, Greece
  • Helen Papadaki
    Department of Internal Medicine, Hematology Clinic of the Univesity Hospital of Heraklion, University of Crete, Medical School, Heraklion, Greece
  • Miltiadis Tsilimbaris
    Department of Neurology and Sensory Organs, Eye CLinic of University Hospital of Heraklion, University of Crete, Medical School, Heraklion, Greece
  • Footnotes
    Commercial Relationships Chrysanthi Tsika, None; Zoi Kapsala, None; Charalampos Pontikoglou, None; Vassiliki Louvari, None; Dimitrios Mamoulakis, None; Helen Papadaki, None; Miltiadis Tsilimbaris, None
  • Footnotes
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Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2424. doi:
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      Chrysanthi Tsika, Zoi Kapsala, Charalampos Pontikoglou, Vassiliki Louvari, Dimitrios Mamoulakis, Helen Papadaki, Miltiadis Tsilimbaris; Endothelial Progenitor Cells (EPCs) Detection In The Peripheral Blood Of Young Diabetic Patients with Type 1 Diabetes Mellitus (T1DM)- A Pilot Study. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2424.

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Abstract

Purpose: To evaluate the presence of EPCs in the peripheral blood of young patients with DM type 1- a pilot study.

Methods: Blood samples were collected and analyzed from 9 patients with DM type 1 and 9 controls. The median age was 17(range:8-30)years old and 19(range 10-30)years old in the control group. The median of DM duration was 12 years (range: 5-30). The presence of Diabetic Retinopathy (DR) was documented with indirect ophthalmoscopy and Fluorescence Angiography, according to the Early Treatment of Diabetic Retinopathy Study (ETDRS) criteria. The EPCs were detected with flow cytometry using the following primary antibodies: anti-CD34, anti-CD133, anti-CD45 and anti-hVEGFR2.

Results: Four out of nine patients had evidence of Diabetic Retinopathy (DR), three mild Non Proliferative DR(NPDR) and one Proliferative DR (PDR). EPCs, defined as CD45-/CD34+/CD133+/VEGFR2+ cells, were detected in 3/9 patients, among whom one was unaffected; one had NPDR and one PDR. The respective percentages of EPCs were: 0.0039%, 0.0035%, and 0.0118%. Finally, circulating EPCs were identified in 1/9 individuals of the control group (0.0098%).

Conclusions: CD45-/CD34+/CD133+/VEGFR2+ EPCs populations were detected in the peripheral blood of this group of young diabetic patients. Not all of them had retinal microangiopathy, while our EPC population was also detected in the control group. The clarification of the presence of EPCs in the peripheral blood early in the disease course could potentially offer significant information regarding the progress of diabetic angiopathy.

Keywords: 499 diabetic retinopathy • 529 flow cytometry  
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