June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Dopamine D1 receptor regulates the functional development of retina
Author Affiliations & Notes
  • Ning Tian
    Ophthalmology & Visual Science, University of Utah, Salt Lake City, UT
  • Quanhua He
    College of Pharmacy, The Ohio State University, Columbus, OH
  • Ping Wang
    Ophthalmology & Visual Science, University of Utah, Salt Lake City, UT
  • Footnotes
    Commercial Relationships Ning Tian, None; Quanhua He, None; Ping Wang, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2498. doi:
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      Ning Tian, Quanhua He, Ping Wang; Dopamine D1 receptor regulates the functional development of retina. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2498.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Dopamine D1 receptor is expressed by multiple subtypes of retinal neurons. The acute effects of dopamine D1 receptor on retinal light responses have been studied extensively. However, little is known about the long-term effects of dopamine D1 receptor on the development of retina. We thought to determine whether dopamine D1 receptor plays roles in the activity-dependent development of mouse retina.

Methods: Mouse retinal light responses were recorded using ERG measurements. Young and adult wild type (WT) mice and mice lacking of dopamine D1 receptor were used to evaluate the roles of dopamine D1 receptor in the development of retina. ERGs were also recorded from mice reared in constant darkness to determine whether dopamine D1 receptor plays critical roles in the activity-dependent development of moue retina. The amplitudes, kinetics and response gains of ERG waveforms are quantitatively analyzed.

Results: 1) The amplitudes of ERG a-wave and OPs of D1-/- mice at the age of P13 are not different from that of age-matched WT mice while the b-wave of D1-/- mice are significantly higher than that of age-matched WT mice. 2) At P30, the amplitudes of ERG a-, b-wave and OPs of D1-/- mice are lower than that of age-matched WT mice and the developmental enhancement of ERG responses, especially the b-wave, is significantly reduced in D1-/- mice. 3) D1-/- mice have enhanced response gain between a- and b-wave but reduced gain between b-wave and OPs at the scotopic range. 4) Dark rearing reduced the amplitudes of all ERG components of WT mice but only a- and b-wave in D1-/- mice. 5) Dark rearing reduced the time-to-peak of a-wave in WT mice but prolonged the time-to-peak of a-wave in D1-/- mice. For the b-wave kinetics, dark rearing reduced the time-to-peak in WT mice significantly more than that in D1-/- mice.

Conclusions: 1) Deletion of dopamine D1 receptor affects the light responsiveness of inner and outer retina differently at different postnatal developmental times, with most significant effect on the developmental enhancement of ERG b-wave. 2) Dopamine D1 receptor seems to preferentially regulate the light-sensitive response gain between bipolar and ganglion/amacrine cells. Light deprivation reduced the amplitudes of b-wave in both WT and D1-/- mice and the amplitudes of OPs in WT but not D1-/- mice. 3) Dopamine D1 receptor also regulates the kinetic of ERG a- and b-wave.

Keywords: 510 electroretinography: non-clinical • 497 development  
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