June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Immunohistochemical identification and characterization of a wide-field amacrine cell type in marmoset retina
Author Affiliations & Notes
  • Felix Weltzien
    Department of Ophthalmology and Save Sight Institute, University of Sydney, Sydney, NSW, Australia
    Australian Research Council Centre of Excellence in Vision Science, University of Sydney, Sydney, NSW, Australia
  • Stefano Di Marco
    School of Medical Sciences, University of Sydney, Sydney, NSW, Australia
  • Dario Protti
    School of Medical Sciences, University of Sydney, Sydney, NSW, Australia
  • Teresa Daraio
    Department of Ophthalmology and Save Sight Institute, University of Sydney, Sydney, NSW, Australia
  • Paul Martin
    Department of Ophthalmology and Save Sight Institute, University of Sydney, Sydney, NSW, Australia
    Australian Research Council Centre of Excellence in Vision Science, University of Sydney, Sydney, NSW, Australia
  • Ulrike Grunert
    Department of Ophthalmology and Save Sight Institute, University of Sydney, Sydney, NSW, Australia
    Australian Research Council Centre of Excellence in Vision Science, University of Sydney, Sydney, NSW, Australia
  • Footnotes
    Commercial Relationships Felix Weltzien, None; Stefano Di Marco, None; Dario Protti, None; Teresa Daraio, None; Paul Martin, None; Ulrike Grunert, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2511. doi:
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      Felix Weltzien, Stefano Di Marco, Dario Protti, Teresa Daraio, Paul Martin, Ulrike Grunert; Immunohistochemical identification and characterization of a wide-field amacrine cell type in marmoset retina. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2511.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Amacrine cells are the most diverse class of retinal neurons. In primates more than 25 different types of amacrine cell have been described. Here, we analysed the morphology, density and distribution pattern of a subset of amacrine cells that express the Ca2+ binding protein secretagogin in the marmoset (Callithrix jacchus) retina.

 
Methods
 

Retinas were either subjected to standard immunohistochemistry or pre-labelled with an antibody specific for secretagogin and subsequently immunopositive cells were injected with DiI. A total of 75 DiI labeled amacrine cells was analysed.

 
Results
 

Secretagogin immunoreactivity was present in wide-field amacrine cells with somata in the inner nuclear layer (79%), as well as displaced amacrine cells with somata in the ganglion cell layer (21%). The cell bodies of regular and displaced amacrine cells together form a regular mosaic suggesting that they form a single population. Secretagogin positive amacrine cells have a peak density of 585 cells/mm2 in central retina, which decreases to 55 cells/mm2 in peripheral retina. We estimate that in the inner nuclear layer secretagogin positive amacrine cells make up 0.3% of the total amacrine cell population in marmoset retina. Co-immunostaining with antibodies against glutamic acid decarboxylase indicate that secretagogin immunopositive cells use GABA as their neurotransmitter. The processes of secretagogin positive amacrine cells were broadly stratified in the middle two thirds of the inner plexiform layer between the dendritic strata formed by cholinergic amacrine cells. DiI injection of individual cells revealed that these cells have dendritic trees, which are decorated with small spines and large varicosities.

 
Conclusions
 

Our results suggest that secretagogin positive cells form a subpopulation of GABAergic amacrine cells, which reveal a close resemblance to the “spiny” amacrine cell described in macaque by Mariani (1990).

  
Keywords: 416 amacrine cells • 419 anatomy • 688 retina  
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