June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Telomere Length of peripheral leukocytes is shortened in Ocular Sarcoidosis patients
Author Affiliations & Notes
  • Ian Thompson
    Immunology, NEI, Bethesda, MD
  • Baoying Liu
    Immunology, NEI, Bethesda, MD
  • H Nida Sen
    Immunology, NEI, Bethesda, MD
  • Bogdan Dumitriu
    Hematology Branch, NHLBI, Bethesda, MD
  • Rodrigo Calado
    Hematology Branch, NHLBI, Bethesda, MD
  • Sima Hirani
    Immunology, NEI, Bethesda, MD
  • Mary Morgan
    Hematology Branch, NHLBI, Bethesda, MD
  • Shayma Jawad
    Immunology, NEI, Bethesda, MD
  • Neal Young
    Hematology Branch, NHLBI, Bethesda, MD
  • Robert Nussenblatt
    Immunology, NEI, Bethesda, MD
  • Footnotes
    Commercial Relationships Ian Thompson, None; Baoying Liu, None; H Nida Sen, None; Bogdan Dumitriu, None; Rodrigo Calado, None; Sima Hirani, None; Mary Morgan, None; Shayma Jawad, None; Neal Young, None; Robert Nussenblatt, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2531. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Ian Thompson, Baoying Liu, H Nida Sen, Bogdan Dumitriu, Rodrigo Calado, Sima Hirani, Mary Morgan, Shayma Jawad, Neal Young, Robert Nussenblatt; Telomere Length of peripheral leukocytes is shortened in Ocular Sarcoidosis patients. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2531.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Chronic inflammatory activity has been associated with leukocyte telomere shortening, and short telomere length may occur with higher frequency in autoimmune diseases including sarcodosis. Tumor necrosis factor - alpha (TNF-alpha), a marker of systemic inflammation, has been reported to promote telomere attrition in lymphocytes through the inhibition of telomerase (hTERT) activity. The aim of this study was to determine if telomere length is shortened in peripheral blood leukocytes of ocular sarcoidosis patients as compared to healthy controls, and to investigate the relationship between lymphocyte telomere length and TNF-a serum level.

Methods: Telomere length of leukocytes was measured by quantitative polymerase chain reaction in 44 ocular sarcoidosis patients, and compared to 300 age-matched healthy control samples. 35 of the 44 ocular sarcoidosis patients and 40 healthy controls had their serum levels of TNF-alpha measured by an enzyme-linked immunosorbent assay. TNF-alpha levels were then correlated with leukocyte telomere lengths.

Results: Ocular sarcoidosis patients demonstrated significantly shorter leukocyte telomere lengths when compared with age-matched controls. There was weak negative correlation between leukocyte telomere length and age ( r = -.24, p = 0.11) in ocular sarcoidosis patients. Very short peripheral blood leukocyte telomere lengths (below the first percentile as compared to age-matched controls) were identified in six out of 44 patients. Ocular sarcoidosis patients did not differ from healthy controls in TNF-alpha serum levels.

Conclusions: Telomere attrition in leukocytes is a plausible cause for abnormal leukocyte cell function in ocular sarcoidosis patients. Functional studies are underway to address this.

Keywords: 557 inflammation • 413 aging • 554 immunohistochemistry  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×