June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
CCR7 Expression Profiles in Conjunctival Biopsies from Seasonal Allergic Conjunctivtis Patients Following Challenge
Author Affiliations & Notes
  • Rose Mathew
    Ophthalmology, Duke University, School of Medicine, Durham, NC
  • Amirah Mohd Zaki
    Ophthalmology, UCL Institute of Ophthalmology, London, United Kingdom
  • Grazyna Galatowicz
    Ophthalmology, UCL Institute of Ophthalmology, London, United Kingdom
  • Virginia Calder
    Ophthalmology, UCL Institute of Ophthalmology, London, United Kingdom
  • Daniel Saban
    Ophthalmology, Duke University, School of Medicine, Durham, NC
    Immunology, Duke University, School of Medicine, Durham, NC
  • Footnotes
    Commercial Relationships Rose Mathew, None; Amirah Mohd Zaki, None; Grazyna Galatowicz, None; Virginia Calder, Allergan Inc (C), Allergan Inc (F); Daniel Saban, Schepens Eye Res Inst, Mass Eye and Ear, (P), Eleven Biotherapuetics (R)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2549. doi:
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      Rose Mathew, Amirah Mohd Zaki, Grazyna Galatowicz, Virginia Calder, Daniel Saban; CCR7 Expression Profiles in Conjunctival Biopsies from Seasonal Allergic Conjunctivtis Patients Following Challenge. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2549.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: It has recently been demonstrated that CCR7-expressing dendritic cells contribute to the immunopathogenesis of allergic conjunctivitis in the mouse model. However, whether CCR7 plays a similar role in the human condition is unknown. The aim of this study was to investigate conjunctival expression of CCR7 following allergen challenge in seasonal allergic conjunctivitis (SAC) patients.

Methods: Human anonymised conjunctival tissue biopsies (3 micron) were obtained from SAC donors (n=7, from all males; age: 18-65 years) at 8 hr post allergen challenge. Control, non-inflamed, conjunctival tissues collected from anonymised human donors (n=5 from 3 males; age: 31-57) were also examined. Donor tissues were collected after obtaining informed consent and Local Research Ethics approval in accordance with the Declaration of Helsinki. Tissue sections were stained for anti-human CCR7 (eBioscience), anti-human neutrophil elastase (DAKO), or isotype-matched control rat IgG2a kappa antibody (eBioscience and visualized with a DAB staining kit (Vectastain). Two independent, masked observers enumerated positively stained cells per biopsy area (at least three fields).

Results: CCR7-expressing cells were detected mainly within the subepithelium and stromal areas. Over 70% of tissues from SAC patients stained positive for CCR7, compared to only 20% from control patients. The numbers of CCR7-expressing cells did not appear to correlate with neutrophil numbers, as the difference in the number of neutrophils between control (mean: 44.0 ± 14.0 cells per biopsy) and SAC (mean 82.9 ± 117.8 cells per biopsy) patients was not statistically significant.

Conclusions: CCR7-expressing cells are detectable in human conjunctival tissues and appear to be upregulated during acute inflammation in SAC.

Keywords: 475 conjunctivitis • 490 cytokines/chemokines • 474 conjunctiva  
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