June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Expression of the neural stem cell marker Hes3 in the rodent and human ocular surface
Author Affiliations & Notes
  • Matina Economopoulou
    Ophthalmology, University Clinic Dresden, Dresden, Germany
  • Jimmy Masjkur
    Department of Internal Medicine (MK3), University Clinic Dresden, Dresden, Germany
  • Frederick Raiskup
    Ophthalmology, University Clinic Dresden, Dresden, Germany
  • Mike Karl
    Center for Regenerative Therapies Dresden, Dresden, Germany
  • Richard Funk
    Department of Anatomy, University Clinic dresden, Dresden, Germany
  • Triantafyllos Chavakis
    Department of Internal Medicine (MK3), University Clinic Dresden, Dresden, Germany
  • Lutz Pillunat
    Ophthalmology, University Clinic Dresden, Dresden, Germany
  • Andreas Androutsellis-Theotokis
    Department of Internal Medicine (MK3), University Clinic Dresden, Dresden, Germany
  • Footnotes
    Commercial Relationships Matina Economopoulou, None; Jimmy Masjkur, None; Frederick Raiskup, None; Mike Karl, None; Richard Funk, None; Triantafyllos Chavakis, None; Lutz Pillunat, None; Andreas Androutsellis-Theotokis, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2570. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Matina Economopoulou, Jimmy Masjkur, Frederick Raiskup, Mike Karl, Richard Funk, Triantafyllos Chavakis, Lutz Pillunat, Andreas Androutsellis-Theotokis; Expression of the neural stem cell marker Hes3 in the rodent and human ocular surface. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2570.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Hairy and Enhancer of Split 3 (Hes3) is a recently established biomarker of neural stem cells in the fetal and adult brain of rodents and primates, including humans. Expression of Hes3 persists during quiescence, allowing the detection of non-proliferating stem cells and precursor cells. Hes3 also identifies putative cancer stem cells in aggressive brain tumors, rendering it a useful biomarker of both normal and cancerous neural stem cells. In this work we address whether Hes3 can also be found on the ocular surface.

Methods: Adult mouse and human eye tissue was prepared for immunohistochemical detection of Hes3 and other markers. Physiological human tissue was obtained from a Cornea bank and pterygium samples were obtained from pterygium excision surgery after patient consent.

Results: The adult rodent and human eye, as well as pterygium samples, contain a population of cells expressing Hes3. In the human eye, Hes3+ cells are found predominantly in the limbus where they physically associate with blood vessels.

Conclusions: Hes3, a recent biomarker of neural stem cells in the adult brain and spinal cord, as well as in brain tumors, is also expressed in the human limbus and in pterygium. Hes3+ cells in the eye also show similarities to those in the brain, in that they maintain tight physical associations with blood vessels. These results suggest that limbal Hes3+ cells may have precursor properties, and that a common mechanism may regulate Hes3+ cells in the brain and the eye.

Keywords: 721 stem cells • 480 cornea: basic science  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×