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Ann Strom, Dennis Cortes, Sara Thomasy, Philip Kass, Vijaykrishna Raghuanthan, Mark Mannis, Christopher Murphy; In Vivo Ocular Imaging of the Anterior Segment of the Canine using High-Resolution Optical Coherence Tomography and Confocal Microscopy. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2592.
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To compare normative data for the dog cornea and conjunctiva obtained using high-resolution Fourier-domain optical coherence tomography (FD-OCT), in vivo confocal microscopy (ICVM) and ultrasound pachymetry (US). To determine in vivo retinal thickness for the dog, using FD-OCT.
The cornea and conjunctiva of 16 eyes from 8 healthy young female intact Beagles were imaged using FD-OCT and IVCM. Central, dorsal paraxial and dorsal perilimbal corneal thickness was also measured using US. Quantitative measures for epithelial thickness of the cornea and conjunctiva (FD-OCT), full thickness of the cornea (FD-OCT and US) and central retinal thickness (FD-OCT) were determined.
The cornea and the corneal epithelium was thinnest centrally and thickest peripherally. Central and perilimbal corneal thickness as measured by FD-OCT was 497.5 ± 30.3 μm vs. 646.1 ± 60.4 μm, respectively. Using FD-OCT, central corneal, perilimbal corneal and conjunctival epithelial thickness was 52.4 ± 7.25 μm, 69.1 ± 7.9 μm and 42.98 ± 6.0 μm, respectively. Central retinal thickness as determined by FD-OCT was 199.4 ± 14.4 μm. IVCM enabled detailed visualization of all corneal elements and quantification of epithelial, nerve fiber, stromal and endothelial cell densities. In agreement with the literature, US pachymetry yielded similar though predictably significantly higher values (p<0.05) than FD-OCT in the central corneal region.
High-resolution FD-OCT and IVCM enabled detailed non-invasive evaluation of in vivo canine anterior segment structures. This study provides normative values with which to evaluate data obtained from dogs with spontaneous corneal diseases as well as induced models of anterior segment disease.
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