June 2013
Volume 54, Issue 15
ARVO Annual Meeting Abstract  |   June 2013
Expression of oxidative markers in laser treated rat lenses
Author Affiliations & Notes
  • Thomas Holm
    Eye dep, Glostrup Hospital, Glostrup, Denmark
  • Leif Johnson
    Eye dep, Glostrup Hospital, Glostrup, Denmark
  • Line Kessel
    Eye dep, Glostrup Hospital, Glostrup, Denmark
  • Footnotes
    Commercial Relationships Thomas Holm, None; Leif Johnson, None; Line Kessel, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 2976. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Thomas Holm, Leif Johnson, Line Kessel; Expression of oxidative markers in laser treated rat lenses. Invest. Ophthalmol. Vis. Sci. 2013;54(15):2976.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: The proposed use of non-invasive laser techniques to treat cataract have produced a need to determine the safety of the eye upon such treatment. We have here determined the altered expression of 7 proteins of the repair and protection mechanisms of the eye, as a response to laser treatment.

Methods: Eleven weeks old Brown Norway rats had their right lens treated with a 445nm laser using 40mW output for 30min. The left eye was used as control. Whole eyes were removed 2 days after laser treatment, fixed in 4% paraformaldehyde over night, and afterwards moved through PBS buffers with increasing sucrose concentration. Eyes were cryo sectioned at 10um and protein expression was visualized using immunohistochemistry on sections from the central part of the eye. Eyes were stained with antibodies for the transcription factors Nf-kB & Nrf2, enzyme subunits xCT & GCLc, the glutathione enzymes GSR & GPx1 and the heavy metal scavenger MT I&II. Enucleated lenses were removed after 2 days and homogenized using a rotor-homogenizer, and used for analysis of glutathione concentration as a response to laser treatment.

Results: Of the two transcription factors, only Nf-κB showed increased expression in lens epithelium. The GSH transporter xCT and the GSH synthesising enzyme GCLc remained unaffected as did the GSH homeostasis enzymes Glutathione Reductase (GSR) and Glutathione Peroxidase 1 (GPx1). The heavy metal scavenger metallothionein (MT I&II) showed a marked increase in the lens epithelium. Glutathione analysis did not show a significant increase (p<0.1) in lenses 2 days after laser treatment.

Conclusions: The main response of the eye to the laser treatment is the activation of Nf-κB which in turn increase the expression of MT I&II. The response indicates that the eye is under oxidative stress during blue light irradiation since Nf-κB is primarily activated under oxidative stress. The lack of increased expression of GSH related proteins follow well the lack of significant increase in glutathione concentration.

Keywords: 578 laser • 634 oxidation/oxidative or free radical damage  

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.