June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Biomechanical changes in Human Corneas after Low and High Intensity Collagen Cross-Linking Treatment measured using Scanning Acoustic Microscopy
Author Affiliations & Notes
  • Ithar Beshtawi
    Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom
  • Riaz Akhtar
    School of Engineering, University of Liverpool, Liverpool, United Kingdom
  • Chantal Hillarby
    School of Clinical and Laboratory Science, The University of Manchester, Manchester, United Kingdom
  • Clare O'Donnell
    Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom
    Optegra, Manchester, United Kingdom
  • Xuegen Zhao
    School of Materials, The University of Manchester, Manchester, United Kingdom
  • Arun Brahma
    Manchester Royal Eye Hospital, Manchester, United Kingdom
  • Fiona Carley
    Manchester Royal Eye Hospital, Manchester, United Kingdom
  • Brian Derby
    School of Materials, The University of Manchester, Manchester, United Kingdom
  • Hema Radhakrishnan
    Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom
  • Footnotes
    Commercial Relationships Ithar Beshtawi, None; Riaz Akhtar, None; Chantal Hillarby, None; Clare O'Donnell, None; Xuegen Zhao, None; Arun Brahma, None; Fiona Carley, None; Brian Derby, None; Hema Radhakrishnan, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3115. doi:
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      Ithar Beshtawi, Riaz Akhtar, Chantal Hillarby, Clare O'Donnell, Xuegen Zhao, Arun Brahma, Fiona Carley, Brian Derby, Hema Radhakrishnan, ; Biomechanical changes in Human Corneas after Low and High Intensity Collagen Cross-Linking Treatment measured using Scanning Acoustic Microscopy. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3115.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To assess and compare the biomechanical properties of postmortem human corneas after low and high intensity collagen cross-linking using scanning acoustic microscopy (SAM).

 
Methods
 

Two groups of five human corneal pairs were included in the study. In group (A), five corneas were treated with low intensity cross-linking (epithelium removed, 0.1% riboflavin applied for 30 minutes following which UV-A irradiation (365 nm, 3mW/cm2) was applied for 30 minutes alongside the riboflavin solution) The contralateral cornea was exposed to riboflavin only, and served as a control. In group (B), five corneas were treated with high-intensity collagen cross-linking (epithelium removed, riboflavin 0.1% solution application for 30 minutes prior to UV-A irradiation (365 nm, 9mW/cm2) for 10 minutes, while the riboflavin was re-applied. The contralateral cornea was exposed to riboflavin only, and served as a control. The biomechanical properties of all corneas were tested using SAM.

 
Results
 

In the low intensity cross-linked corneas (group A), the speed of sound of the treated corneas was 1674.51 ± 21.38 ms-1 anteriorly and 1598.24 ± 27.83 ms-1 posteriorly, while it was 1593.96 ± 22.51 ms-1 anteriorly and posteriorly 1578.64 ± 18.83 ms-1 in the untreated corneas. In the high-intensity cross-linked corneas (group B), the speed of sound of the treated corneas was 1663.55 ± 20.16 ms-1 anteriorly and 1593.17 ± 20.30 ms-1 posteriorly, while it was 1582.38 ± 15.30 ms-1 anteriorly and 1564.12 ± 18.46 ms-1 posteriorly in the untreated corneas. The speed of sound is directly proportional to the stiffness of the tissue. Therefore the results indicate that corneal stiffness changes are similar in the low and high intensity cross-linking groups.

 
Conclusions
 

Scanning acoustic microscopy is a novel tool for examining the biomechanical properties of human corneal tissue. It was used successfully to assess corneal stiffness after applying collagen cross-linking. Similar results were observed in corneas treated with low and high intensity cross-linking protocols.

 
 
The anterior and posterior (200 x 200) µm portion of low intensity cross-linked (A and B, respectively) and untreated (C and D respectively) corneal sections.
 
The anterior and posterior (200 x 200) µm portion of low intensity cross-linked (A and B, respectively) and untreated (C and D respectively) corneal sections.
 
 
The anterior and posterior (200 x 200) µm portion of high intensity cross-linked (A and B, respectively) and untreated (C and D respectively) corneal sections.
 
The anterior and posterior (200 x 200) µm portion of high intensity cross-linked (A and B, respectively) and untreated (C and D respectively) corneal sections.
 
Keywords: 480 cornea: basic science • 678 refractive surgery  
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