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Manabu Hirasawa, Motoyoshi Endo, Mamoru Kamoshita, Seiji Miyake, Toshio Narimatsu, Norihiro Nagai, Kazuo Tsubota, Yoko Ozawa; Involvement of Angiopoietin-like 2 in Laser-induced Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2013;54(15):319.
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Recent studies has revealed that angiopoietin-like 2 (Angptl2) has an important role in angiogenesis, chronic inflammation, and macrophage infiltration. Although these changes are involved in the pathogenesis of age-relaeted macular degeneration (AMD), little is known about Angptl2 in AMD. In this study we investigated expression of angptl2 during generation of laser-induced choroidal neovascularization (CNV) in mice.
Male C57BL/6 mice were anesthetized and CNV was induced by laser-photocoagulation. Mice were sacrificed one, two, or three days after laser injury, and eye samples were obtained. Immunohistochemistry for RPE65 (RPE specific marker) and F4/80 (macrophage specific marker) were performed with nuclear counter staining with Hoechst 33258. Real-time PCR was performed using the whole retinal pigment epithelium (RPE)-choroid complex sample to examine the mRNA levels of Angptl2, and its receptors, integrins α5, β1, and pirb, which were normalized to gapdh.
In the laser-induced CNV model, Angptl2 was localized in the RPE and macrophages. Increase in the mRNA levels of integrins α5, β1, and pirb were all obvious in the whole RPE-choroid complex of this model, although the increase in Angple2 was not detected, in the early phase of CNV generation.
The current data proposed the possibility of Angptl2 signal’s involvement via integrin and/or pirb in the pathogenesis of CNV. Further study will unravel the role of Angptl2 in the CNV generataion.
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