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Hiroki Kaneko, Ryo Ijima, Shu Kachi, Hiroko Terasaki; Anti-histamine receptor 4 therapy inhibits choroidal neovascularization in mice. Invest. Ophthalmol. Vis. Sci. 2013;54(15):338.
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© ARVO (1962-2015); The Authors (2016-present)
To examine the therapeutic ability of anti-histamine receptor 4 (H4R) treatment against choroidal neovascularization (CNV) in mice.
JNJ7777120, neutralizing chemical against H4R, was commercially purchased and resuspended with DMSO/PBS. Laser-induced CNVs were performed in the eyes of 6 week-old male C57Bl/6J mice, followed by intravitreous injection of 1ug JNJ7777120. The eyes injected with DMSO/PBS after laser-CNVs were used as control. Seven days after laser and intravitreous injection, eyes were fixed with 4% paraformaldehyde, and stained with isolection B4. CNV volumes were analyzed using confocal microscope (Nikon). H4r expressions were also measured by western blotting in the retina, and retinal pigment epithelium (RPE)/choroid complex 3 days after inducing laser-CNVs.
CNV volume in the eyes injected with JNJ7777120 was smaller compared with those injected with DMSO/PBS. Furthermore, the H4r protein expression in the retina was reduced by inducing laser-CNV, whereas H4r expression in RPE/choroid complex was not reduced.
The treatment against H4R had the possibility to reduce CNV in wet age-related macular degeneration. However, precise mechanism is not clear. To elucidate remaining questions, further study will be required.
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