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Mélanie Bidaut-Garnier, Mathieu Flores, Perle TUMAHAI, meillat mathieu, Guillaume Debellemaniere, Marc Puyraveau, Bernard Delbosc, Michel Montard, Maher Saleh; Reproducibility of photoreceptor imaging by Adaptive Optics Retinal Camera. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3440.
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© ARVO (1962-2015); The Authors (2016-present)
To report the reproducibility and the repeatability of photoreceptor imaging in healthy human eyes, using the RTx-1™ Adaptive Optics (AO) Retinal Camera, (Imagine Eyes, Orsay, France) and AOdetect v0.1 cone counting software.
Three healthy emmetropic adults of 30, 42 and 42 years old, were imaged. Mean cone density (cells/mm2) at a fixed distance from the fovea (0.3 x 0.3 degree square located at 1° temporally), spacing between cells (µ) and percentage of hexagonal cells calculated on Voronoi diagrams, were also measured using the proprietary software of the device. Intersession repeatability was assessed by imaging both eyes of each subject, 5 consecutive days, by the same operator. Intrassession repeatability was assessed by comparing 10 consecutive acquisitions, obtained by the same operator from the right eye of each subject. Inter-operator variability was evaluated by comparing the images acquired by three different operators. The cone counts were blind measured by 2 graders using AOdetect v0.1. Pearson Coefficient (r) and coefficients of variation (COVs) were calculated.
The mean cone counts were 25475 ± 2983/mm2 and 26396 ±3174 cells/mm2 for the first and the second grader, respectively (t test, p <0.05). All the parameters evaluated were highly reproducible: Pearson's r reached 0.90 (CI 95%: 0.84; 0.94) between both graders and 0.96 (CI 95%:0.57 ; 0.96) for the inter-operator variability. The intra-session and intersession COVs were 6% and 4%, respectively. The measurements of percentage of hexagonal cells and spacing between cells also varied in the same proportion (COV range from 3 to 9 %).
Photoreceptor counts using the RTx-1™ Adaptive Optics Retinal Camera showed good reproducibility and repeatability in normal human eyes. Further studies on the use of AO in the normal clinical setting are needed.
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