June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Corneal infection induces migration of dendritic cells with CD1a expression
Author Affiliations & Notes
  • Mari Narumi
    Opthalmology & Vis Sciences, Yamagata Univ Faculty of Med, Yamagata, Japan
  • Yoshiko Kashiwagi
    Department of health and nutrition, Yamagata prefectural yonezawa women's junior college, Yamagata, Japan
  • Hiroyuki Namba
    Opthalmology & Vis Sciences, Yamagata Univ Faculty of Med, Yamagata, Japan
  • Ritaro Ohe
    Department of Diagnostic Pathology, Yamagata University Faculty of Medicine, Yamagata, Japan
  • Mitsunori Yamakawa
    Department of Diagnostic Pathology, Yamagata University Faculty of Medicine, Yamagata, Japan
  • Hidetoshi Yamashita
    Opthalmology & Vis Sciences, Yamagata Univ Faculty of Med, Yamagata, Japan
  • Footnotes
    Commercial Relationships Mari Narumi, None; Yoshiko Kashiwagi, None; Hiroyuki Namba, None; Ritaro Ohe, None; Mitsunori Yamakawa, None; Hidetoshi Yamashita, Senju (C), Senju (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3668. doi:
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      Mari Narumi, Yoshiko Kashiwagi, Hiroyuki Namba, Ritaro Ohe, Mitsunori Yamakawa, Hidetoshi Yamashita; Corneal infection induces migration of dendritic cells with CD1a expression. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3668.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Dendritic cells (DCs) migrate to the central part of infected cornea, of which mechanisms have not been fully clarified. We analyzed the factors relevant to the migration including the patients’ clinical backgrounds, and also investigated the chemotactic factors of DCs in cornea with pathological states.

Methods: The present study was approved by the Ethical Committee of Yamagata University Faculty of Medicine. We obtained surgically 22 corneal tissues, and blood for migration assay from 2 healthy volunteers. We observed immunohistochemically the expression of specific DC markers (CD1a, DC-SIGN, CD83, Langerin, S-100 protein) and also the numbers of newly formed vessels and lymphatic vessels with CD31 and D2-40, and the densities of DCs. We investigated statistically the factors relevant to the distributions of each DCs. The factors were the states of corneal infection, perforation, history of previous penetrating keratoplasty (PKP), and newly formed lymphatic vessels and/or blood vessels. To investigate the migration mechanisms, immature monocyte derived DCs (immoDCs) were isolated from blood and CD1a was used as a marker. The migration was observed using Boyden’s chamber method with TNF-α,TGF-β1, IL-6 as chemotactic factor candidates.

Results: The lymphatic vessels were detected in 15, 10 with newly formed vessels, and the vessel formation was correlated with the infection and/or the perforation and/or the PKP. Immature DCs (CD1a+ DC) were mainly observed in the corneal epithelial and the sub-epithelial layers. The density of CD1a + DCs increased significantly in the cases with corneal infection and/or perforation. DCs with S-100 protein were increased in the cases with infection and/or with newly formed vessels. Lymphatic vessels were observed only in the cases with infection, and were not correlated with the DC distribution. The migration assay showed that TNF-α and IL-6 (100ng/ml each) were chemotactic factors for immoDCs, and TGF-β1 was less effective.

Conclusions: The states of infection, perforation, neovascularization may affect the distributions of the corneal DCs, especially the CD1a + DCs in the central part. The inflammatory cytokines accelerated the migration of CD1a + DCs. Taken together, the corneal pathological states above shown may accelerate the migration of immature DCs by inflammatory cytokines, which may be correlated with formation of blood vessels and lymphatic vessels.

Keywords: 480 cornea: basic science • 554 immunohistochemistry • 490 cytokines/chemokines  
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