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Catherine Garcher, Marie-Noelle Delyfer, Marie Rougier, Hélène Savel, Geneviève Chêne, Cecile Delcourt, Jean-Francois Korobelnik; Comparison of macular pigment optical density measured by autofluorescence and reflectometry: the LIMPIA Study. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3781.
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To compare macular pigment optical density (MPOD) measured by two techniques (autofluorescence, reflectometry) in healthy subjects at high risk for age-related macular degeneration (AMD).
The Limpia Study is a double-blind, placebo controlled, prospective randomized clinical trial performed in 120 subjects with at least one parent affected by neovascular AMD. To be included, subjects had to be aged 40-70 years, have best-corrected visual acuity (BCVA) greater than 20/25, be free of late AMD and other major eye conditions (severe glaucoma, high myopia, severe retinal disease, cataract surgery…). Subjects having used supplements containing lutein and/or zeaxanthin in the preceding year were not included. MPOD was measured using two methods: the two-wavelength autofluorescence method with a modified scanning laser ophthalmoscope (Heidelberg Retinal Analyzer (HRA), Heidelberg, Germany) and a method based on reflectometry (Visucam200 MPD, Carl ZeissMeditec, Germany).
At baseline, mean MPOD within 0.51°, measured with the modified HRA method, was 0.5 ± 0.2. Maximal MPOD, measured with Visucam, was 0.4 ± 0.1. Parameters from the modified HRA (optical density within 0.5°, 1°, 2° and 6°) and from the Visucam method (volume, area, mean and maximum optical density) were weakly correlated. The best correlations were observed for MPOD within 2° and 6° with volume from Visucam (r=0.21 and r=0.22, respectively), and mean MPOD from Visucam (r=0.21 and r=0.18).
The two methods propose different parameters to evaluate macular pigment, which overall correlated weakly in this population of middle-aged healthy subjects at high risk for AMD. Further research is needed to characterize the differences between the methods and identify the best parameters and techniques to measure macular pigment.
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