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Jin-Wook Jang, Hansoo Kim, Chan-Young Cho, Jinkuk Kim, Ju-Woong Jang, Young-Sik Kim, Ynag-je Cho; Application of eye drops for inhibiting scar formation using BMP-7 with excipients. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3880.
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The purpose of this study is to applicate BMP-7 for inhibiting scar formation eye drop with excipients.
1. Effect of BMP-7 with excipients in HaCaT cells: The expression levels of TGF-beta induced fibronectin and alpha-SMA in HaCaT cells were measured. HaCaT cells were split 6 well plates. 2 days after, the cells were serum-starved. Then 5 ng/ml of TGF-beta and 200 ng/ml of BMP-7 with excipients (Hyaluronic acid, Chitosan and Cyclodextrin) were treated and incubated for 24 hours. The supernatants were collected, TGF-beta induced fibronectin and alpha-SMA were measured by western blot and ELISA. 2. Cell viability influence of BMP-7 with excipients: Cell viability was measured cell viability assay kit based on colormetric assay. HaCaT cells were cultured in a 96-well plate for 24 hours. Then BMP-7 only and BMP-7 with excipients were treated for 48 hours. After treatments, 10 μl of Kit reagent put into each well and incubated for 1 hour. Finally, optical density of 450 nm was measured by plate reader.
TGF-β induced fibronectin and α-SMA expression is reduced by BMP-7 with Hyaluronic acid, Chitosan and Cyclodextrin in HaCaT cells. The excipients did not interfere with effects of BMP-7 and also did not affect cell viability.
BMP-7 with excipients has the ability to reduce fibrosis and also the excipients contributed to the stabilization of BMP-7. As such, it seems possible that this technology can be applied to eye drops and become a new innovative treatment in pharmaceutical markets.
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