June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
CD11b+GR1+ Myeloid Cells Promote Trigeminal Ganglion Neurite Growth: Implications for Corneal Nerve Regeneration
Author Affiliations & Notes
  • Sonal Gandhi
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Joy Sarkar
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Wallace Chamon
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Shweta Chaudhary
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Sapna Tibrewal
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Yong-Soo Byun
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Sarmad Jassim
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Abhishek Sharma
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Neil Mohindra
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Sandeep Jain
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Footnotes
    Commercial Relationships Sonal Gandhi, None; Joy Sarkar, None; Wallace Chamon, None; Shweta Chaudhary, None; Sapna Tibrewal, None; Yong-Soo Byun, None; Sarmad Jassim, None; Abhishek Sharma, None; Neil Mohindra, None; Sandeep Jain, PCT/US20/51562 (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 3884. doi:
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      Sonal Gandhi, Joy Sarkar, Wallace Chamon, Shweta Chaudhary, Sapna Tibrewal, Yong-Soo Byun, Sarmad Jassim, Abhishek Sharma, Neil Mohindra, Sandeep Jain, ; CD11b+GR1+ Myeloid Cells Promote Trigeminal Ganglion Neurite Growth: Implications for Corneal Nerve Regeneration. Invest. Ophthalmol. Vis. Sci. 2013;54(15):3884.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: It is becoming increasingly clear that the nervous and inflammatory (myeloid) systems display considerable overlap in their molecular and cellular repertoire. Bone marrow-derived YFP+ cells infiltrate the cornea during nerve regeneration in thy1-YFP mouse. We characterized these cells and determined whether they promote trigeminal ganglion (TG) cell neurite growth.

Methods: Excimer laser annular keratectomy was performed in thy1-YFP+ mice and corneas were imaged to visualize regenerating nerves and infiltrating cells. Bone marrow cells (BMCs) were harvested from naive thy1-YFP mice and a flow cytometry analyzer was used to assess the expression levels of cell surface markers in the total BMC population. Compartmental cultures of dissociated TG cells were performed and sorted YFP+ cells (>95% pure) were co-cultured in the side compartments with neurites to determine their neurotrophic effect.

Results: Following annular keratectomy, YFP+ cells infiltrate the cornea and localize adjacent to transected nerves. These are bone marrow-derived cells that share surface markers (CD11b+Gr1+Ly6C+Ly6G-F4/80low) with monocytic myeloid-derived suppressor cells (MDSCs); thus, we call them YFP-MDSCs. They are CD11c, CD3e and MHC-II negative. YFP-MDSCs significantly increase the growth of TG neurites and make physical contact with neurites. When cultured in a transwell with TG neurites, YFP-MDSCs express neurotrophins and secrete NGF and BDNF in the conditioned media.

Conclusions: CD11b+Gr1+ myeloid cells promote neurite growth in TG cells. These cells infiltrate the cornea after nerve transection surgery and interact with the regenerating nerves; thus they may promote reinnervation by their neurotrophic action. One mechanism by which these cells promote neurite growth is by secretion of neurotrophins.

Keywords: 480 cornea: basic science • 687 regeneration  
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