June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Parafoveal retinal vascular response to pattern stimulation assessed with OCT angiography
Author Affiliations & Notes
  • Eric Wei
    Casey Eye Institute, Oregon Health & Science University, Portland, OR
  • Yali Jia
    Casey Eye Institute, Oregon Health & Science University, Portland, OR
  • Ou Tan
    Casey Eye Institute, Oregon Health & Science University, Portland, OR
  • Benjamin Potsaid
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA
    Advanced Imaging Group, Thorlabs, Inc., Newton, NJ
  • Jonathan Liu
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA
  • WooJhon Choi
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA
  • James Fujimoto
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA
  • David Huang
    Casey Eye Institute, Oregon Health & Science University, Portland, OR
  • Footnotes
    Commercial Relationships Eric Wei, None; Yali Jia, Optovue Inc (P); Ou Tan, Optovue (F), Optovue (P), Carl Zeiss Meditec (P); Benjamin Potsaid, Thorlabs, Inc. (E), Optovue, Inc. (P); Jonathan Liu, None; WooJhon Choi, None; James Fujimoto, Carl Zeiss Meditec (P), Optovue (P), Optovue (I); David Huang, Optovue (F), Optovue (I), Optovue (P), Optovue (R), Carl Zeiss Meditec (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 399. doi:
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    • Get Citation

      Eric Wei, Yali Jia, Ou Tan, Benjamin Potsaid, Jonathan Liu, WooJhon Choi, James Fujimoto, David Huang; Parafoveal retinal vascular response to pattern stimulation assessed with OCT angiography. Invest. Ophthalmol. Vis. Sci. 2013;54(15):399.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To measure the parafoveal retinal vascular response to stimulation with a reversing checkerboard pattern using OCT angiography.

 
Methods
 

One eye of each subject was scanned with an ultra-high speed (100,000 A-scans/sec) 1050 nm wavelength swept-source OCT device. Each 3x3 mm OCT angiography image, comprised of a 200x200 scan pattern with 8 repeated B-scans at each position, was acquired in 3.4 seconds. Flow was detected from the decorrelation of signal amplitude between repeated B-scans using the split-spectrum amplitude-decorrelation angiography (SSADA) algorithm. Parafoveal retinal flow index was calculated from en face maximum projections of the retinal layers by averaging decorrelation values within the parafoveal region defined by a 1 mm wide annulus surrounding the fovea avascular zone (FAZ). A reversing checkerboard pattern modulated at 8 cycles/second was used to stimulate the macula. The experimental sequence, performed twice for each subject, consisted of 1 minute of baseline, 2 minutes with stimulation on, and 1 minute of stimulation off, with scans of the macula captured in 15 second intervals.

 
Results
 

Five normal subjects were evaluated. An increase of 6.09 ± 4.69% (mean ± standard deviation of 5 subjects) over baseline (p = 0.001, paired t-test) was observed during the first minute of stimulation and 1.96 ± 5.39% (p = 0.275) during the second minute. Following cessation of the pattern stimulus, a 0.40 ± 6.04% increase in flow index over baseline was observed (p = 0.896). The most significant change to flow index was seen 30 seconds into stimulation where a 7.12% ± 4.35% increase from baseline occurred (p < 0.001) (Fig. 1). The repeatability of the 4 consecutive baseline measurements within the same sequence was 1.3% coefficient of variation (CV). The repeatability between 2 baseline sequences was 2.1% CV.

 
Conclusions
 

OCT angiography with SSADA is able to detect increased flow in the parafoveal retinal vasculature in response to pattern stimulation. The response was large relative to the repeatability of baseline flow index measurements. However, the magnitude of the response was highly variable between individuals.

 
 
Figure 1. False color representation of en face retinal angiograms show increased flow (higher decorrelation values) in the angiogram captured 30 seconds into stimulation (middle) compared to baseline (left) and 30 seconds after stimulation was turned off (right).
 
Figure 1. False color representation of en face retinal angiograms show increased flow (higher decorrelation values) in the angiogram captured 30 seconds into stimulation (middle) compared to baseline (left) and 30 seconds after stimulation was turned off (right).
 
Keywords: 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 585 macula/fovea • 436 blood supply  
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