June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Light-induced translocation of RGS9-1 and Gβ5L in Mouse Rod Photoreceptors
Author Affiliations & Notes
  • You-Wei Peng
    Genetics, Boys Town Natl Research Hosp, Omaha, NE
  • Mei Tian
    Genetics, Boys Town Natl Research Hosp, Omaha, NE
  • Marisa Zallocchi
    Genetics, Boys Town Natl Research Hosp, Omaha, NE
  • Weimin Wang
    Genetics, Boys Town Natl Research Hosp, Omaha, NE
  • Ching-Kang Chen
    Biochemistry & Molecular Biology, Virginia Commonwealth University, Richmond, VA
  • Krzysztof Palczewski
    Pharmacology, Case Western Reserve University, Cleveland, OH
  • Dominic Cosgrove
    Genetics, Boys Town Natl Research Hosp, Omaha, NE
  • Footnotes
    Commercial Relationships You-Wei Peng, None; Mei Tian, None; Marisa Zallocchi, None; Weimin Wang, None; Ching-Kang Chen, None; Krzysztof Palczewski, QLT Inc (F), Polgenix Inc (E), Visum Inc (P), Amegen Inc (F); Dominic Cosgrove, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 4078. doi:
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      You-Wei Peng, Mei Tian, Marisa Zallocchi, Weimin Wang, Ching-Kang Chen, Krzysztof Palczewski, Dominic Cosgrove; Light-induced translocation of RGS9-1 and Gβ5L in Mouse Rod Photoreceptors. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4078.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The transducin GTPase-accelerating protein complex, which determines the photoresponse duration of photoreceptors, is composed of RGS9-1, Gβ5L and R9AP. Here we report that RGS9-1 and Gβ5L change their distribution in rods during light/dark adaptation.

Methods: Immunohistochemistry, serial tangential section immunoblotting, immunoprecipitation and immunoblotting using anti-Phospho-RGS9 antibodies were used to study the light-induced protein translocation.

Results: Upon prolonged dark adaptation, RGS9-1 and Gβ5L are primarily located in rod inner segments. But very dim-light exposure quickly translocates them to the outer segments. In contrast, their anchor protein R9AP remains in the outer segment at all times. In the dark, Gβ5L’s interaction with R9AP decreases significantly and RGS9-1 is phosphorylated at S475 to a significant degree. Dim light exposure leads to quick de-phosphorylation of RGS9-1. Furthermore, after prolonged dark adaptation, RGS9-1 and transducin Gα are located in different cellular compartments.

Conclusions: These results suggest a previously unappreciated mechanism by which prolonged dark adaptation leads to increased light sensitivity in rods by dissociating RGS9-1 from R9AP and redistributing it to rod inner segments

Keywords: 648 photoreceptors • 646 phosphorylation • 689 retina: distal (photoreceptors, horizontal cells, bipolar cells)  
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