June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
In vivo Evaluation of Surface Modified Expanded-polytetrafluroethylene (ePTFE) Substrate as a Permanent Substrate for Cell Transplantation
Author Affiliations & Notes
  • Shen Nian
    Ophthalmology, University of Hong Kong, Hong Kong, Hong Kong
  • Zhongjie Fu
    Ophthalmology, University of Hong Kong, Hong Kong, Hong Kong
  • Carl Sheridan
    Eye and Vision Science, University of Liverpool, Liverpool, United Kingdom
  • Victoria Kearns
    Eye and Vision Science, University of Liverpool, Liverpool, United Kingdom
  • Rachel Williams
    Eye and Vision Science, University of Liverpool, Liverpool, United Kingdom
  • Sai Hung David Wong
    Ophthalmology, University of Hong Kong, Hong Kong, Hong Kong
  • Krasimir Vasilev
    Mawson Institute and School of Advanced Manufacturing, University of South Australia, Mawson Lakes, SA, Australia
  • Akash Bachhuka
    Mawson Institute and School of Advanced Manufacturing, University of South Australia, Mawson Lakes, SA, Australia
  • Amy Lo
    Ophthalmology, University of Hong Kong, Hong Kong, Hong Kong
    Research Centre of Heart, Brain, Hormone and Healthy Aging, University of Hong Kong, Hong Kong, Hong Kong
  • Wico Lai
    Ophthalmology, University of Hong Kong, Hong Kong, Hong Kong
  • Footnotes
    Commercial Relationships Shen Nian, None; Zhongjie Fu, None; Carl Sheridan, None; Victoria Kearns, None; Rachel Williams, None; Sai Hung David Wong, None; Krasimir Vasilev, None; Akash Bachhuka, None; Amy Lo, None; Wico Lai, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 4085. doi:
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      Shen Nian, Zhongjie Fu, Carl Sheridan, Victoria Kearns, Rachel Williams, Sai Hung David Wong, Krasimir Vasilev, Akash Bachhuka, Amy Lo, Wico Lai; In vivo Evaluation of Surface Modified Expanded-polytetrafluroethylene (ePTFE) Substrate as a Permanent Substrate for Cell Transplantation. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4085.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Age-related macular degeneration (AMD) is the leading cause of severe and permanent visual loss in patients above 55 years of age with no effective treatments. Transplantation of a functional cell monolayer grown on the substrate into the subretinal space may help rescue the photoreceptors and in turn treating AMD. However, degradation of biodegradable substrates may cause the breakdown of functional cell monolayer and produce toxic byproducts. Therefore, synthetic non-degradable materials have been employed as the permanent underlying substrate to establish an intact functional cell monolayer for subretinal transplantation. We aimed to investigate the host response of surface modified ePTFE substrates after subretinal transplantation.

Methods: Fibronectin coated n-heptylamine modified (F-HA) ePTFE substrate was transplanted into the subretinal space of 4-week-old Royal College of Surgeons (RCS) rat eyes. Fundus was photographed and intraocular pressure (IOP) was measured immediately before and after surgery and weekly thereafter until RCS rats were sacrificed. 1 and 4 weeks after surgery, eyes were isolated and examined by H&E-stained retinal sections. Inflammatory responses were evaluated by immunostaining of tumor necrosis factor α (TNFα) and interleukin-1β (IL1β). Glial cell responses were assessed by immunostaining of glial fibrillary acidic protein (GFAP).

Results: Retinal detachment was only observed immediately after surgery in fundus photos. IOP of rat eyes remained at about 10 mmHg except a drop to 7 mmHg immediately after transplantation. Retinal morphology was similar with/without substrate transplantation. Expression of pro-inflammatory cytokines (TNFα and IL1β) and GFAP was not significantly increased in the F-HA ePTFE substrate-transplanted eyes at 1 and 4 weeks. The F-HA ePTFE substrates remained flat beneath the neural retina up to 4 weeks after transplantation. Some cells were found to attach to F-HA ePTFE substrates and were positively stained with isolectin but negatively with RPE65.

Conclusions: F-HA ePTFE substrate showed good biocompatibility and lack of host inflammatory and glial cell responses, suggesting that it is a suitable permanent underlying substrate for subretinal transplantation.

Keywords: 741 transplantation • 412 age-related macular degeneration  
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