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Bianca Maceo, Fabrice Manns, Alberto de Castro, Stephen Uhlhorn, Esdras Arrieta, Susana Marcos, Jean-Marie Parel; Lens Spherical Aberration Changes in Cynomolgus Monkeys during Simulated Accommodation in a Lens Stretcher. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4272.
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To quantify the difference in spherical aberration (SA) of cynomolgus monkey lenses in the accommodated and unaccommodated state.
A Laser Ray Tracing system (LRT) was used to obtain SA measurements on 2 cynomolgus monkey lenses from 2 donors (4.5 and 6.9 years, PMT= 4 and 26 hours). The tissue was mounted in a chamber filled with Dulbecco's Modified Eagle Medium inside a lens stretcher (Ehrmann et al, Clin Exp Opt, 2008). The lens spherical aberration was measured in the unstretched (accommodated) and stretched (relaxed) state. The LRT delivered 51 equally-spaced parallel rays along the vertical meridian of the lens over a total length of 6mm. A camera mounted on a vertical position stage was placed under the chamber containing the lens to sequentially record the spot corresponding to each individual ray. Spot images were measured at up 12 camera positions along the optical axis covering a range of up to 22mm. The images were processed to determine the centroid of each spot and calculate the corresponding ray height at each camera position. For each camera position, the measured ray heights were plotted as a function of entrance ray height and fit with a 3rd order polynomial: y = A*x + B*x3. The A and B coefficients were then plotted as a function of camera position and a linear fit was performed. The effective focal length and Seidel spherical aberration coefficient were extracted from the fits. A ray-trace analysis showed that the posterior window of the chamber contributes less than 1% error to the SA coefficient. Therefore the contribution of the window to spherical aberration was not corrected for.
The results for the two lenses are summarized in the table below:
Spherical aberration in cynomolgus monkey lenses increases in absolute value with accommodation, as found in human and rhesus monkeys.
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