June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
The Effect of a Temperature-Sensitive Poloxamer/Alginate/CaCl2 Mixture After Strabismus Surgery in a Rabbit Model
Author Affiliations & Notes
  • Won Yeol Ryu
    Department of Ophthalmology, Dong-A University College of Medicine, Busan, Republic of Korea
  • Hye Mi Jung
    Department of Ophthalmology, Dong-A University College of Medicine, Busan, Republic of Korea
  • Yoon Hyung Kwon
    Department of Ophthalmology, Dong-A University College of Medicine, Busan, Republic of Korea
  • Woo Park
    Department of Ophthalmology, Dong-A University College of Medicine, Busan, Republic of Korea
  • Hee-Bae Ahn
    Department of Ophthalmology, Dong-A University College of Medicine, Busan, Republic of Korea
  • Footnotes
    Commercial Relationships Won Yeol Ryu, None; Hye Mi Jung, None; Yoon Hyung Kwon, None; Woo Park, None; Hee-Bae Ahn, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 4716. doi:
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      Won Yeol Ryu, Hye Mi Jung, Yoon Hyung Kwon, Woo Park, Hee-Bae Ahn; The Effect of a Temperature-Sensitive Poloxamer/Alginate/CaCl2 Mixture After Strabismus Surgery in a Rabbit Model. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4716.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To determine the efficacy of a temperature-sensitive poloxamer/alginate mixture in reducing adhesions after strabismus surgery in a rabbit model.

Methods: Thirty-six eyes of 18 rabbits were randomly divided into two groups. After the recession of the superior rectus muscle (SRM) in one eye of each rabbit, the poloxamer/alginate mixture was applied under and on top of the SRM (PA group). As a control, normal saline was injected subconjunctivally into the other eye of each rabbit. The adhesions among the SRM, sclera, and conjunctiva were evaluated. Inflammation was analyzed by H&E staining and the anti-CD11b antibody. Late fibrosis was assessed by Masson’s trichrome and α-smooth muscle actin antibodies. Adhesion, inflammation, and fibrosis were graded on a scale of 0 to 4.

Results: Comparing the degree of adhesions between the groups at 1 day and 4 weeks after surgery, there was no statistically significant difference. However, the degree of adhesions in the PA group was lower than that of the control group at postoperative week 1 (p=0.030). Acute inflammation exhibited no statistically significant difference between both groups (p>0.05). At postoperative week 4, the infiltrated inflammatory cells were significantly reduced in the PA group (p=0.027). A significant difference was observed in late fibrosis between the groups by Masson’s trichrome and α-smooth muscle actin antibodies (p=0.033, p=0.022, respectively).

Conclusions: The poloxamer/alginate mixture significantly decreased the adhesions after strabismus surgery in a rabbit model. Additional studies are warranted to evaluate the use of this mixture in other ophthalmic surgeries in preventing postoperative adhesions.

Keywords: 725 strabismus: treatment • 765 wound healing  
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