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Anum Butt, Sung Chul Park, Rafael Furlanetto, Wendy Kirkland, Camila Netto, Mohammed Al-Jumayli, Jeffrey Liebmann, Robert Ritch; Enhanced Depth Imaging Optical Coherence Tomography of Trabecular Outflow Pathway. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4831.
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© ARVO (1962-2015); The Authors (2016-present)
To assess the usefulness of enhanced depth imaging optical coherence tomography (EDI OCT) for evaluating in vivo microarchitecture of the trabecular outflow pathway.
Serial horizontal EDI OCT B-scans were obtained in the nasal and temporal limbal areas from one eye of normal subjects (81 scans per 15x5 degree rectangle; interval between scans, ~35 µm; Fig A and B). The intra- and inter-observer reproducibility of the Schlemm’s canal (SC) cross-sectional area measurement was evaluated using 40 randomly selected EDI OCT scans (8 scans per each of 5 normal eyes). Analysis was based on 3 independent series of re-evaluations made by two observers. The microarchitecture of SC and collector channel (CC) was assessed. For one eye, serial EDI OCT was performed in a radial fashion in every clock hour and 3-dimensional image of the entire SC circumference was reconstructed.
Eleven normal eyes (11 subjects; mean age, 28±5 years) were included. The SC cross-sectional area measurement by the two observers showed excellent intra- (ICC [intraclass correlation coefficient] = 0.830 for observer 1 and 0.886 for observer 2) and inter-observer (ICC = 0.793) reproducibility (all p<0.001). SC was continuous in the scanned area in all eyes. The cross-sectional shape of SC varied considerably among regions with intermittent bifurcation (Fig C-E). Most CCs were connected to the outer wall of the SC and their locations varied from the medial to the lateral ends of SC (Fig F-H). The entire SC circumference was successfully reconstructed 3-dimensionally (Fig I and J).
EDI OCT is useful for evaluating the in vivo microarchitecture of the trabecular outflow pathway. This has implications for the development of new drugs and techniques to reduce intraocular pressure.
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