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Woo Jin Jeong, Michael Abramoff, Bhavna Antony, Chunhua Jiao, Mona Garvin, Elliott Sohn; Retinal thickness changes in mice with streptozotocin-induced diabetes mellitus quantified using an enhanced Iowa Reference Algorithm. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4883.
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© ARVO (1962-2015); The Authors (2016-present)
Inner retinal thinning in humans with type I diabetes mellitus (DM) has been previously described by us and others in eyes with no detectable diabetic retinopathy. In this study we sought to compare retinal topography in vivo of wild type mice to those with DM using the Iowa Reference Algorithm for three-dimensional automated layer segmentation of spectral domain optical coherence tomography (OCT).
C57BL/6 mice (3 months) were assigned into streptozotocin-induced type 1 DM (n=10) and age-matched control cohorts (n=8). Retinal OCT imaging was performed (Bioptigen, Morrisville, NC) system at baseline and 6 weeks after induction of DM. Using the Iowa Reference Algorithm, mathematically enhanced for mouse OCT, average change in thickness of the combined retinal nerve fiber layer and inner plexiform layer (RNFL-IPL) and total retinal thickness were quantified and compared using two-tailed paired t-test analysis at 0 and 6 weeks for each group.
Mean total retinal thickness of the DM group at 6 weeks (135.6μm) was thinner (p=0.02) compared to baseline (137.6μm; 95% CI, 1.29-5.71μm). The mean total retinal thickness of the control group at 6 weeks (137.3μm) was similar to baseline (137.9μm). Mean thickness of RNFL-IPL layer in the DM and control groups was similar at 6 weeks and baseline.
The Iowa Reference Algorithm was successfully enhanced for mouse OCT 3D segmentation, and a significant decrease in total retinal thickness was quantified in mice after 6 weeks of streptozocin-induced diabetes mellitus was not attributable to changes in inner retinal thickness. On-going research is underway to determine the precise retinal layers responsible for the attenuation.
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