June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Spectral-Domain Optical Coherence Tomography of the Optic Nerve Head in a Canine Model of Primary Open-Angle Glaucoma
Author Affiliations & Notes
  • Annie Oh
    College of Veterinary Medicine, Michigan State University, East Lansing, MI
    College of Veterinary Medicine, Western University of Health Sciences, Pomona, CA
  • Christine Harman
    College of Veterinary Medicine, Michigan State University, East Lansing, MI
  • Kristin Koehl
    College of Veterinary Medicine, Michigan State University, East Lansing, MI
  • Jiayan Huang
    Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA
  • Leandro Teixeira
    School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI
  • Laurence Occelli
    College of Veterinary Medicine, Michigan State University, East Lansing, MI
  • Eric Storey
    School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA
  • Gui-Shuang Ying
    Scheie Eye Institute, University of Pennsylvania, Philadelphia, PA
  • Andras Komaromy
    College of Veterinary Medicine, Michigan State University, East Lansing, MI
    School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA
  • Footnotes
    Commercial Relationships Annie Oh, None; Christine Harman, None; Kristin Koehl, None; Jiayan Huang, None; Leandro Teixeira, None; Laurence Occelli, None; Eric Storey, None; Gui-Shuang Ying, None; Andras Komaromy, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 4893. doi:
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      Annie Oh, Christine Harman, Kristin Koehl, Jiayan Huang, Leandro Teixeira, Laurence Occelli, Eric Storey, Gui-Shuang Ying, Andras Komaromy; Spectral-Domain Optical Coherence Tomography of the Optic Nerve Head in a Canine Model of Primary Open-Angle Glaucoma. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4893.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Inherited primary open-angle glaucoma (POAG) in Beagle dogs is a well established large animal model of glaucoma, and it is caused by a G661R missense mutation in the ADAMTS10 gene. This study represents the first attempt to describe early glaucomatous optic neuropathy by spectral-domain optical coherence tomography (SD-OCT).

Methods: The optic nerve heads (ONHs) of 9 adult, glaucomatous dogs (mean age +/- SD: 43 +/- 2.5 months; mean diurnal intraocular pressure (IOP): 29.9 +/- 1.1 mmHg) and 3 related, age-matched control dogs (IOP: 18.0 +/- 1.3 mmHg) were imaged under general anesthesia by SD-OCT (Spectralis®, Heidelberg Engineering). All of the dogs were sighted with normal retinal function as recorded by flash electroretinography. The thickness of the peripapillary retina was measured at 8 locations, and ONH dimensions were evaluated in the horizontal and vertical planes. OCT measurements were compared between affected and control dogs by ANOVA. The correlations of OCT measurements with IOP and axial globe length were assessed by Spearman correlation coefficient. OCT images from affected and control dogs were compared qualitatively with selected histologic ONH sections.

Results: There were no significant differences in the thickness of any retinal layers evaluated between glaucomatous (mean full retinal thickness: 239 +/- 6.0 μm) and normal control dogs (243 +/- 3.2 μm). Compared to histologic cross-sections, OCT images showed that the normal canine ONH protruded more noticeable into the vitreous. Of all the ONH parameters evaluated, the loss of neuroretinal rim height in the horizontal plane was most significant (from 154 +/-38.4 μm to 9.3 +/- 22.1 μm; p < 0.01). Furthermore, there was a highly significant negative correlation between neuroretinal rim height and IOP (Spearman correlation: -0.86; p<0.0001). Even though the cupping of the ONH dramatically worsened in a few affected dogs, the average cup dimensions were not significantly different between glaucomatous and normal control dogs.

Conclusions: SD-OCT is a useful tool to detect early glaucomatous optic neuropathy in dogs. The decrease in neuroretinal rim height without inner retinal thinning and without obvious functional loss suggests that initial degenerative changes are mostly due to loss of myelin.

Keywords: 472 comparative anatomy • 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 629 optic nerve  
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