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Joseph Ho, Mehreen Adhi, Emily Cole, Jonathan Liu, James Fujimoto, Jay Duker, Nadia Waheed; Reproducibility and Agreeability of Automated Retinal Pigment Epithelial Detachment Volumetric Measurements via Spectral Domain Optical Coherence Tomography. Invest. Ophthalmol. Vis. Sci. 2013;54(15):4914.
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© ARVO (1962-2015); The Authors (2016-present)
To assess the agreeability and reproducibility of retinal pigment epithelial detachment (PED) volume and area measurements utilizing a commercially available optical coherence tomography (OCT) software available for the Cirrus HD-OCT.
Patients with a diagnosis of dry or wet age-related macular degeneration (AMD), with evidence of PED between October and December 2012 were eligible. All patients were imaged using the macular cube 512x128 scan protocol. Each study eye was imaged three times with each scan spaced 5 minutes apart. ‘Retinal pigment epithelium (RPE) elevation analysis’ software was used to automatically calculate the PED volumes and areas in the 3 mm and 5 mm circles from the foveal center. AMIRA software was used to manually calculate PED volumes for each patient. Interclass correlation coefficients (ICCs) were calculated to assess the reproducibility of the automated PED volumes and areas among the three scans and then between the automated and manual measurements. The incidence of automated segmentation line breakdown for both the ‘RPE fit’ and the ‘true RPE’ lines in the central 1 mm region of the macula was also evaluated for error analysis.
12 eyes from 10 patients were included, 8 with dry AMD and 4 with wet AMD. The average age was 72 years, with 8 females and 2 males. Average PED via automated segmentation was 0.14 mm3 in the central 3 mm circle and 0.21 mm3 in the 5 mm circle. Average automated PED area was 2.37 mm2 in the central 3 mm circle and 3.77 mm2 in the 5 mm circle. Manual segmentation yielded an average PED volume of 0.50 mm3 in the 3 mm and 0.92 mm3 in the 5 mm circles. The ICCs across the three automated measurements were 0.954 for the volume in the 3 mm circle, 0.983 for the volume in the 5 mm circle, 0.971 for the area in the 3 mm circle, and 0.992 for the area in the 5 mm circle. The ICC between the manual and automatic volume is 0.033 for the 3 mm circle and 0.296 for the 5 mm circle. In the center 1 mm region, 11 of the 12 scans demonstrated ‘RPE fit’ line breakdown, while 8 of the 12 scans showed ‘true RPE’ line breakdown.
RPE analysis software allowed for reproducible calculation of PED volumes and areas. Agreeability between manual and automated volumetric measurements was low secondary to a high incidence of segmentation line breakdown.
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