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Won Seok Choi, Eui Sang Chung, Tae-Young Chung, Joon Young Hyon, Won Ryang Wee, Young Joo Shin; ROCK Inhibitor Y-27632 Enhances the Mucin Secretion from Goblet Cells Derived from Limbal Stem Cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5410.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the effect of Rho-associated coiled kinase inhibitor (ROCK inhibitor) Y-27632 on Goblet cells derived from corneal limbal stem cells.
Goblet cells were derived from human limbal stem cells and cultured. The cells were treated with ROCK inhibitor. Periodic acid-Schiff (PAS) staining and immunofluorescence staining of ABCG2, CK-7 and MUC5AC was performed. Cell proliferation rate was measured with BrdU labeling assay.
The cultured cells were stained with ABCG2 and CK-7. Goblet cell derived from limbal stem cells showed mucin secretion by PAS staining and MUC5AC staining. Cell proliferation rate increased with ROCK inhibitor.
Goblet cells were differentiated from limbal stem cells. ROCK inhibitor enhanced mucin secretion and proliferation rate in Goblet cells derived from limbal stem cells.
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