June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
The role of reactive oxygen species on cytotoxicity of moxifloxacin and benzalkonium chloride on human corneal epithelial cells
Author Affiliations & Notes
  • Ta-Ching Chen
    National Taiwan University Hospital, Taipei, Taiwan
  • Fung-Rong Hu
    National Taiwan University Hospital, Taipei, Taiwan
  • Footnotes
    Commercial Relationships Ta-Ching Chen, None; Fung-Rong Hu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5414. doi:
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      Ta-Ching Chen, Fung-Rong Hu; The role of reactive oxygen species on cytotoxicity of moxifloxacin and benzalkonium chloride on human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5414.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigate the role of reactive oxygen species (ROS) and its mechanism on cytotoxicity of moxifloxacin and benzalkonium chloride (BAK) on human corneal epithelial cells (HCECs).

Methods: Cultured HCECs were incubated with 0.5% moxifloxacin as unpreserved solutions, and 0.001% benzalkonium chloride (BAC) for 4 periods (5 min, 30 min, 1 h, and 3 h). Drug effects on cell viability were evaluated by trypan blue exclusion assay and MTS assay. Levels of reactive oxygen species (ROS) production were evaluated using luminol- and lucigenin-enhanced chemiluminescence assay. The protective effect of anti-oxidants (N-acetylcysteine and 3,5,4'-trihydroxy-trans-stilbene) on cytotoxicity of the tested drugs was evaluated by MTS assay.

Results: A significant decrease in cell viability was observed following incubation with 0.001% BAC and 0.5% moxifloxacin. Cell recovery test revealed poor reversibility of cytotoxicity in both moxifloxacin and BAC groups after more than 30 minutes of exposure. ROS production was significantly increased after incubation of HCECs in moxifloxacin and BAC solutions. Pre-treatment of anti-oxidants may provide some protective effects toward ROS-related cytotoxicity.

Conclusions: The cytotoxicity of moxifloxacin and BAK was related to ROS production. Anti-oxidants offer protective effect toward ROS-related cytotoxicity.

Keywords: 482 cornea: epithelium • 480 cornea: basic science  
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