June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Decreased Phosphorylation at the Serine-2 Residue of the RNA Polymerase II in Corneal Epithelial Stem Cells
Author Affiliations & Notes
  • Satoshi Kawasaki
    Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • Katsuhiko Shinomiya
    Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • Keita Aoi
    Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
    Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan
  • Shigeru Kinoshita
    Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • Footnotes
    Commercial Relationships Satoshi Kawasaki, None; Katsuhiko Shinomiya, None; Keita Aoi, None; Shigeru Kinoshita, Senju Pharmaceutical Co (P), Santen Pharmaceutical Co (P), Otsuka Pharmaceutical Co (C), Alcon (R), AMO (R), HOYA (R)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 558. doi:
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      Satoshi Kawasaki, Katsuhiko Shinomiya, Keita Aoi, Shigeru Kinoshita; Decreased Phosphorylation at the Serine-2 Residue of the RNA Polymerase II in Corneal Epithelial Stem Cells. Invest. Ophthalmol. Vis. Sci. 2013;54(15):558.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Continuous regeneration of stem cells is known to be essential for maintaining the life-long homeostasis of any types of cells in the body of animals. In the stem cells of ocular surface epithelia, several positive and negative markers have been reported. Recent studies have shown that stem cells are adapted to hypoxic conditions with slow cell cycling, low transcriptional activity, lower mitochondrial respiration, and higher glycolysis for the generation of adenosine-5'-triphosphate (ATP). The purpose of this present study was to report a new marker for corneal epithelial stem cells based on low transcriptional activity.

Methods: Corneal tissue samples obtained from humans, rabbits, and mice were cryo-embedded, sliced into thin sections, fixed, and immunostained with antibodies against the phosphorylated serine-2 of the RNA polymerase II (RNAP II), a marker of activated transcription, as well as several positive or negative markers of corneal epithelial stem cells such as N-cadherin, p63, and keratin 12.

Results: In all of the tissue samples, a decreased level of phosphorylation at the serine-2 residue of the RNAP II was found at the basal cells of the limbal epithelium. Immunostaining with antibodies against the above-described established markers for corneal epithelial stem cells proved that those cells were, in fact, corneal epithelial stem cells.

Conclusions: The findings of this study show that phosphorylated serine-2 of the RNAP II is a new negative marker for corneal epithelial stem cells. Corneal epithelial stem cells may have a general feature of stem cells in terms of low transcriptional activity.

Keywords: 721 stem cells • 738 transcription  
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