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Takashi Fujishiro, Makoto Aihara, Chihiro Mayama, Makoto Araie; Analysis of retinal ganglion cell in lateral geniculate nucleus using ferret ocular hypertension model. Invest. Ophthalmol. Vis. Sci. 2013;54(15):56.
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© ARVO (1962-2015); The Authors (2016-present)
Ferrets have binocular vision unlike mice and rats, therefore ferrets are more suitable for analyzing the central visual pathway damage caused by glaucoma compared to mice and rats. Recent studies reported that not only optic nerve but also lateral geniculate nucleus (LGN) and visual cortex were damaged by glaucoma. We reported the ferret ocular hypertension model last year at ARVO 2012 and analyze the damage of the central visual pathway in this model. Retinal ganglion cells (RGCs) are divided into three cell lines which are known for magno, parvo and konio cells in primates and the corresponding ones in the ferrets are defined as X, Y and W cells. It is reported that magno cells are damaged first, then parvo cells are damaged in early stage glaucoma in primates. However, there are few reports about the damage of konio cells in early stage glaucoma for now because of difficulty of analysis of konio cells in LGN. W cells in ferret may be analyzed easily because of being different from structure of LGN in primates. We analyzed X, Y and W cells in LGN using ferret ocular hypertension model.
We established ferret ocular hypertension model by injecting conjunctival cells into an anterior chamber. Fluorescent labeled cholera toxin B (CTB) was injected to the vitrous space at 3 months after ocular hypertension. Red CTB was injected into the right eye and green CTB was injected into the left eye. Brain stems were removed from the skull of ferrets in 4 days after CTB injection. Damage of X, Y and W cells in LGN was investigated using fluorescent microscope
Elevation of IOP was occurred and sustained for 3 months after the injection of conjunctival cells. Fluorescence intensity in ocular hypertension model ferret was apparently decreased not only in contralateral LGN projected from eyes of elevated IOP but also in ipsilateral LGN projected from eyes of normal IOP compared to the normal ferret. Histologically, W cells are more damaged than X and Y cells in LGN.
W cells might be more sensitive than W and Y cells in ferret ocular hypertension model. Ferret ocular hypertension model will be a useful tool to investigate glaucomatous optic neuropathy and the secondary damage in the visual pathway.
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