June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Resting Potential of Rat Crystalline Lens
Author Affiliations & Notes
  • Sebastiao Cronemberger
    Ophthalmology, Federal University of Minas Gerais, Belo Horizonte, Brazil
  • Vinicius Oliveira
    Ophthalmology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
  • Renata Fleming
    Ophthalmology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
  • Nassim Calixto
    Ophthalmology, Federal University of Minas Gerais, Belo Horizonte, Brazil
  • Adalmir Dantas
    Ophthalmology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
  • Footnotes
    Commercial Relationships Sebastiao Cronemberger, None; Vinicius Oliveira, None; Renata Fleming, None; Nassim Calixto, None; Adalmir Dantas, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5731. doi:
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    • Get Citation

      Sebastiao Cronemberger, Vinicius Oliveira, Renata Fleming, Nassim Calixto, Adalmir Dantas; Resting Potential of Rat Crystalline Lens. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5731.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To measure the resting potential (RP) in the anterior and posterior surfaces of the rat crystalline lens (CL) investigating if there is difference between the center and the four quadrants.

 
Methods
 

We performed 20 experiments (10 for each surface) on rat CL. Immediately after decapitation the eyes were removed and the posterior pole and vitreous were excised. The CL was placed in a modified Ringer solution (RS) with the cornea downwards. To measure the RP of the anterior surface of the CL the cornea was removed. The temperature in the chamber was set at 30°C by means of a thermostatic bath. RS composition in mM was Na+Cl- 100, K+Cl- 6, Ca++Cl- 2, MgSO4 1, NaHPO4 1, NaHCO3 30 and glucose 20. The membrane potential was measured using a microelectrode that was inserted inside the CL through the posterior or anterior pole at the center and at the four peripheral quadrants. The microelectrode was connected via voltmeter to a reference electrode that was immersed in RS. The signal (mV) was amplified and displayed and registered on a Grass polygraph. The external electrode is outside the CL capsule. When the microelectrode crosses the CL capsule, it sends a signal that is recorded as a negative potential in relation to the potential measured by the reference electrode. The difference between the values of the two electrodes gives the RP. The statistical analysis was made by Graph Pad Prism 6.0.

 
Results
 

The mean, standard deviation and standard error of the RP of the rat CL are on the Table 1 below.

 
Conclusions
 

The RP of the center and the four quadrants of the anterior and posterior surfaces of the rat CL ranged from 40 to 58 mV with no statistically significant difference.

 
 
Table 1: Mean, standard deviation and standard error of the RP of the rat CL.
 
Table 1: Mean, standard deviation and standard error of the RP of the rat CL.
 
Keywords: 488 crystallins • 445 cataract • 507 electrophysiology: clinical  
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