June 2013
Volume 54, Issue 15
ARVO Annual Meeting Abstract  |   June 2013
Microfiltration of Brilliant Blue G Dye
Author Affiliations & Notes
  • Sri Krishna Mukkamala
    Ophthalmology, Columbia University, New York, NY
    Vitreous Retina Macula Consultants, New York, NY
  • Footnotes
    Commercial Relationships Sri Krishna Mukkamala, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5775. doi:
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    • Get Citation

      Sri Krishna Mukkamala; Microfiltration of Brilliant Blue G Dye. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5775.

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      © ARVO (1962-2015); The Authors (2016-present)

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Brilliant Blue G (BBG) is a safe and effective dye used to highlight the internal limiting membrane during macular surgery. A recent outbreak of BBG associated Fusarium spp. endophthalmitis raises concerns about this off-label, non- FDA approved intraocular use. We propose that the utilization of a 0.22 μm filter intraoperatively can reduce the risk of inoculating an eye with contaminated BBG.


An in vitro model of contaminated BBG was prepared. Laboratory stock cultures of 7 organisms including Staphyloccocus epidermidis, Streptococcus pneumoniae, Staphyloccocus aureus, Haemophilus influenza, Klebsiella pneumoniae, Fusarium spp., and Candida albicans were prepared in five 10-fold dilutions and injected into BBG vials. These mixtures were drawn with either a 5 μm filter, 0.22 μm, or without a filter and cultured on appropriate plates and growth conditions.


No culture plates that had inoculate drawn through a 0.22 μm filter showed evidence of growth. There was evidence of growth for all organisms when no filter was used. A 5 μm was insufficient to filter Fusarium spp.


Using a 0.22 μm filter in the intraoperative processing of BBG would likely reduce the risk of infectious endophthalmitis resulting from contaminated dye.

Keywords: 762 vitreoretinal surgery • 467 clinical laboratory testing  

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