Purchase this article with an account.
Won-Mo Lee, Jae Yon Won, Ji-Sun Paik, Won-Kyung Cho, Eun-Hye Oh, Seong-Beom Lee, Suk-Woo Yang; Rapamycin enhances the TNF-α-induced secretion of IL-6 and IL-8 by suppressing PDCD4 degradation in orbital fibroblasts. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5907.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate the effects of rapamycin on the TNF-α-induced secretion of interleukin (IL)-6 and IL-8 in orbital fibroblasts and its possible mechanism.
Orbital fibroblasts were obtained from patients with thyroid-associated ophthalmopathy. IL-6 and IL-8 levels were measured by enzyme-linked immunosorbent assays. The down-regulation of PDCD4 (program cell death 4) was performed by PDCD4 siRNA transfection.
Rapamycin significantly enhanced TNF-α-induced IL-6 and IL-8 secretion from orbital fibroblasts. Down-regulation of PDCD4 by PDCD4 siRNA transfection reduced TNF-α-induced IL-6 and IL-8 secretion from orbital fibroblasts. In addition, TNF-α was found to promote the mTOR-dependent proteasome-mediated degradation of PDCD4. Rapamycin increased PDCD4 expression via the inhibition of TNF-α-induced PDCD4 degradation in orbital fibroblasts.
Rapamycin enhances the TNF-α-induced secretion of IL-6 and IL-8 by suppressing PDCD4 degradation in orbital fibroblasts.
This PDF is available to Subscribers Only