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Anne Kasus-Jacobi, Gina Griffith, Megan Lerner, H Pereira; Cytokine Profiling in Mouse Cornea During Wound Healing in Response to CAP37 Treatment. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5994.
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The cationic antimicrobial protein CAP37 mediates proliferation, migration, and adhesion of cultured human corneal epithelial cells (HCEC) and promotes corneal wound healing in the mouse. The purpose of this study was to investigate using multiplex analysis which cytokines were affected during wounding of the cornea, and to identify those cytokines modulated by treatment with CAP37 to determine the mechanism whereby CAP37 contributes to the recruitment of inflammatory cells and healing of the cornea.
In a mouse model of corneal wound healing, a 2 mm diameter abrasion of the corneal epithelium was created utilizing the Algerbrush® II. Wounds were treated at 0 and 16 h with the full length human recombinant CAP37 (250 and 500 ng/ml), or the vehicle. Wounds were visualized with fluorescein staining at 0, 16 and 24 h, and measured using ImageJ software. Re-epithelialization was assessed by histology, infiltration of inflammatory cells was analyzed by immunohistochemistry, and the cytokine profile was investigated by multiplex analysis.
An accelerated wound closure was found in corneas treated with rCAP37. Re-epithelialization of the corneal epithelium in rCAP37 treated wounds was complete by 24h. Immunohistochemistry revealed greater neutrophil infiltration in treated than untreated wounded corneas. Monocyte chemoattractant protein-1 (MCP-1), granulocyte colony-stimulating factor (GCSF), keratinocyte-derived cytokine (KC), interferon gamma-induced protein 10 (IP-10), leukemia inhibitory factor (LIF), and interleukins 5 (IL-5) and 9 (IL-9) were found to be induced by wounding and were modulated by CAP37 treatment. In general, CAP37 appeared to differentially modulate the chemokines involved in monocyte and neutrophil migration.
These data demonstrate that CAP37 facilitates accelerated corneal wound healing in vivo and modulates the release of cytokines in the cornea. The recruitment of inflammatory cells in corneal wound healing remains controversial. These findings suggest that recruitment of neutrophils maybe critical for corneal reepithelialization during the acute phase, implying that CAP37 has the potential for use as a therapeutic for corneal injuries.
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