June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Comparison of Gene Expression Profile of Epiretinal Membranes obtained from Eyes with Proliferative Vitreoretinopathy to that of Secondary Epiretinal Membranes
Author Affiliations & Notes
  • Shigeo Yoshida
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Ryo Asato
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Takahito Nakama
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Keijiro Ishikawa
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Shintaro Nakao
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Yukio Sassa
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Hiroshi Enaida
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Yuji Oshima
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Tatsuro Ishibashi
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Footnotes
    Commercial Relationships Shigeo Yoshida, None; Ryo Asato, None; Takahito Nakama, None; Keijiro Ishikawa, None; Shintaro Nakao, None; Yukio Sassa, None; Hiroshi Enaida, None; Yuji Oshima, None; Tatsuro Ishibashi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 6250. doi:
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      Shigeo Yoshida, Ryo Asato, Takahito Nakama, Keijiro Ishikawa, Shintaro Nakao, Yukio Sassa, Hiroshi Enaida, Yuji Oshima, Tatsuro Ishibashi; Comparison of Gene Expression Profile of Epiretinal Membranes obtained from Eyes with Proliferative Vitreoretinopathy to that of Secondary Epiretinal Membranes. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6250.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Proliferative vitreoretinopathy (PVR) is a destructive complication of retinal detachment and vitreoretinal surgery which can lead to severe vision reduction by tractional retinal detachments. The purpose of this study was to determine the gene expression profile of epiretinal membranes (ERMs) associated with a PVR (PVR-ERM) and to compare it to the expression profile of less-aggressive secondary ERMs.

Methods: A PCR-amplified complementary DNA (cDNA) library was constructed using the RNAs isolated from ERMs obtained during vitrectomy. The sequence from the 5' end was obtained for randomly selected clones and used to generate expressed sequence tags (ESTs).

Results: We obtained 1116 nonredundant clusters representing individual genes expressed in PVR-ERMs, and 799 clusters representing the genes expressed in secondary ERMs. The transcriptome of the PVR-ERMs was subdivided by functional subsets of genes related to metabolism, cell adhesion, cytoskeleton, signaling, and other functions, by FatiGo analysis. The genes highly expressed in PVR-ERMs were compared to those expressed in the secondary ERMs, and these were subdivided by cell adhesion, proliferation, and other functions. Querying 10 cell adhesion-related genes against the STRING database yielded 70 possible physical relationships to other genes/proteins, which included an additional 60 genes that were not detected in the PVR-ERM library. Of these, soluble CD44 and soluble vascular cellular adhesion molecule-1 were significantly increased in the vitreous of patients with PVR.

Conclusions: Our results support an earlier hypothesis that a PVR-ERM, even from genomic points of view, is an aberrant form of wound healing response. Genes preferentially expressed in PVR-ERMs may play an important role in the progression of PVR and could be served as therapeutic targets.

Keywords: 655 proliferative vitreoretinopathy • 688 retina • 533 gene/expression  
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