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Keijiro Ishikawa, Shigeo Yoshida, Shintaro Nakao, Takahito Nakama, Takeshi Kita, Yukio Sassa, Hiroshi Enaida, Yuji Oshima, Toshihiro Kono, Tatsuro Ishibashi; Periostin Promotes the Generation of Fibrous Membranes in Proliferative Vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6253.
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© ARVO (1962-2015); The Authors (2016-present)
We previously reported the increased expression of periostin in patients with proliferative vitreoretinopathy (PVR) and that periostin enhanced the proliferation, adhesion, migration and collagen synthesis through integrin αV-mediated FAK and AKT phosphorylation of retinal pigment epithelial (RPE) cells. The purpose of this study was to investigate, in vivo, the suppressive effect of periostin on the pathogenesis of fibrous membrane with PVR.
We generated mouse PVR model using C57BL/6 WT and periostin knockout mice according to the previous report. Masson’s trichrome staining and immunofluorescence staining with collagen type I antibody were performed to evaluate the formation of the fibrous membranes. We observed the effect of periostin neutralizing Ab (NAb) on rabbit PVR model that involves injecting RPE cells in the vitreous. Electroretinography and histological examination were performed to evaluate the retinal function after intravitreal injections of periostin NAb.
The findings of Masson’s trichrome staining showed scarce collagen deposition in the lesions of periostin knockout mice compared to WT mice. The areas of fibrous membrane were significantly decreased in the RPE/choroidal flat mount preparations in periostin knockout mice compared to WT mice (p <0.05). Administration of periostin NAb significantly suppressed the progression of PVR from day 5 to day 21. The mean amplitude of the photopic b wave in the rabbit eye treated with periostin NAb was not significantly different from that of the control eye. The histological structure of the rabbit retina in the eyes injected with periostin NAb appeared normal.
The present study identified periostin as a promising therapeutic target for PVR.
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