June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
AQUAPORIN-1 EXPRESSION IN MEMBRANES FROM PROLIFERATIVE VITREORETINOPATHY AND IN EPIRETINAL MEMBRANES
Author Affiliations & Notes
  • Laure Caspers
    Ophthalmology, CHU Saint-Pierre Hospital - ULB, Brussels, Belgium
  • Elie Motulsky
    Ophthalmology, CHU Saint-Pierre Hospital - ULB, Brussels, Belgium
    Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles (ULB), Brussels, Belgium
  • Dany Salik
    Ophthalmology, CHU Saint-Pierre Hospital - ULB, Brussels, Belgium
    Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles (ULB), Brussels, Belgium
  • Xavier Janssens
    Ophthalmology, CHU Saint-Pierre Hospital - ULB, Brussels, Belgium
    Ophthalmlogy, Saint-Anne Saint-Rémi Hospital, Brussels, Belgium
  • Bart Pion
    Ophthalmology, CHU Saint-Pierre Hospital - ULB, Brussels, Belgium
    Ophthalmlogy, Saint-Anne Saint-Rémi Hospital, Brussels, Belgium
  • Nargis Bolaky
    Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles (ULB), Brussels, Belgium
  • Francoise Gregoire
    Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles (ULB), Brussels, Belgium
  • Jason Perret
    Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles (ULB), Brussels, Belgium
  • Francois Willermain
    Ophthalmology, CHU Saint-Pierre Hospital - ULB, Brussels, Belgium
    I.R.I.B.H.M, Université Libre de Bruxelles (ULB), Brussels, Belgium
  • Christine Delporte
    Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles (ULB), Brussels, Belgium
  • Footnotes
    Commercial Relationships Laure Caspers, None; Elie Motulsky, None; Dany Salik, None; Xavier Janssens, None; Bart Pion, None; Nargis Bolaky, None; Francoise Gregoire, None; Jason Perret, None; Francois Willermain, None; Christine Delporte, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 6256. doi:
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      Laure Caspers, Elie Motulsky, Dany Salik, Xavier Janssens, Bart Pion, Nargis Bolaky, Francoise Gregoire, Jason Perret, Francois Willermain, Christine Delporte; AQUAPORIN-1 EXPRESSION IN MEMBRANES FROM PROLIFERATIVE VITREORETINOPATHY AND IN EPIRETINAL MEMBRANES. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6256.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: As Aquaporin-1 (AQP1) is involved in cell migration and proliferation, the purpose of the study was to investigate its expression in membranes from proliferative vitreoretinopathy (PVR) and epiretinal membranes (ERM).

Methods: 19 Membranes from PVR and ERM were collected following eye surgery. AQP1 mRNA and protein expression were determined by RT-qPCR and immunofluorescence in both PVR and ERM.

Results: AQP1 mRNA and protein were expressed in both PVR and ERM as shown by RT-qPCR and immunofluorescence. However, AQP1 protein expression was heterogeneous among and between PVR and ERM. AQP1 was colocalized with alpha-smooth muscle actin (αSMA or Acta2) and colocalized with glial fibrillary acidic protein (GFAP). All the markers used including AQP1, GFAP and αSMA were all statistically positively correlated. All the correlation coefficients calculated were all statistically significant except the correlation coefficient calculated between GFAP and GFAP+AQP1.

Conclusions: AQP1 mRNA and protein were expressed in both PVR and ERM. Due to the absence of αSMA and GFAP colocalization, it is likely that AQP1 is expressed by at least two distinct cells types. AQP1 might play a role in cell migration and proliferation occurring during the formation of PVR and ERM. Therefore, AQP1 could represent a new therapeutic target.

Keywords: 655 proliferative vitreoretinopathy • 701 retinal pigment epithelium • 688 retina  
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